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pC-GoldyTALEN
(Plasmid #38143)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38143 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBSK
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5702
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GoldyTALEN
  • Alt name
    TALEN
  • Species
    Synthetic
  • Insert Size (bp)
    1800
  • Mutation
    Truncate at N and C terminous
  • Promoter miniCaggs
  • Tags / Fusion Proteins
    • FokI homodimer (C terminal on insert)
    • AcV5 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Esp31 (destroyed during cloning)
  • 3′ cloning site Esp31 (destroyed during cloning)
  • 5′ sequencing primer CGTGACCGCAATGGAGGC
  • 3′ sequencing primer CACTGCATCCATGGCAGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A derivative of pTAL-Leu, a TALEN plasmid submitted by the Dan Voytas lab.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899).

For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC-GoldyTALEN was a gift from Daniel Carlson (Addgene plasmid # 38143)
  • For your References section:

    Efficient TALEN-mediated gene knockout in livestock. Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC. Proc Natl Acad Sci U S A. 2012 Oct 1. 10.1073/pnas.1211446109 PubMed 23027955