Purposedestination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38142||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 4651
Vector typemRNA transcription vector
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1800
MutationTruncate at N and C terminous
- Promoter T3
/ Fusion Proteins
- FokI homodimer (C terminal on insert)
- AcV5 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Esp31 (destroyed during cloning)
- 3′ cloning site Esp31 (destroyed during cloning)
- 5′ sequencing primer CGTGACCGCAATGGAGGC
- 3′ sequencing primer CACTGCATCCATGGCAGG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byIt is a deriviative of a plasmid (pTAL-Leu) in the TALEN kit supplied by Dan Voytas.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Please note that this plasmid can be prone to recombination in bacteria. We recommend storing and propagating the construct in Stbl3 cells and screen 4-6 colonies by sequencing with TAL_F1 (ttggcgtcggcaaacagtgg) primer.
The F1 origin feature in the full plasmid sequence is in reverse compliment orientation. Please view Addgene's quality control sequence for the most accurate information. The flipped orientation does not affect the SacI site necessary to linearize the plasmid.
For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899).
For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCIscript-GoldyTALEN was a gift from Daniel Carlson & Stephen Ekker (Addgene plasmid # 38142 ; http://n2t.net/addgene:38142 ; RRID:Addgene_38142)
For your References section:Efficient TALEN-mediated gene knockout in livestock. Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC. Proc Natl Acad Sci U S A. 2012 Oct 1. 10.1073/pnas.1211446109 PubMed 23027955