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High-sensitivity measurements of multiple kinase activities in live single cells.
Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
59138pENTR-ERKKTRCloverORF encoding ERK KTR mClover flanked by Gateway sequences
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59139pENTR-JNKKTRCloverORF encoding JNK KTR mClover flanked by Gateway sequences
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59142pENTR-p38KTRCloverORF encoding p38 KTR mClover flanked by Gateway sequences
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59146pENTR-PKAKTRCloverORF encoding PKA KTR mClover flanked by gateway sequences
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59148pENTR-JNKKTRmRuby2ORF encoding JNK KTR mRuby2 flanked by Gateway sequences
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59149pENTR-p38KTRmCerulean3ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences
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59150pLentiCMV Puro DEST ERKKTRCloverLentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)
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59151pLentiPGK Puro DEST JNKKTRCloverLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)
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59152pLentiPGK Puro DEST p38KTRCloverLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)
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59153pLentiPGK Puro DEST PKAKTRCloverLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)
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59154pLentiPGK Blast DEST JNKKTRmRuby2Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)
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59155pLentiCMV Puro DEST p38KTRmCerulean3Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)
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