PurposeORF encoding JNK KTR mRuby2 flanked by Gateway sequences
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59148||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert nameJNK Kinase Translocation Reporter
SpeciesH. sapiens (human), M. musculus (mouse)
Entrez GeneMAPK8 (a.k.a. JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c)
/ Fusion Protein
- mRuby2 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer CCTTTTTGCGTTTCTACA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR-JNKKTRmRuby2 was a gift from Markus Covert (Addgene plasmid # 59148 ; http://n2t.net/addgene:59148 ; RRID:Addgene_59148)
For your References section:High-sensitivity measurements of multiple kinase activities in live single cells. Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW. Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039. 10.1016/j.cell.2014.04.039 PubMed 24949979