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169,450 results

Showing: 3641 - 3660 of 169450 results

  1. TFORF0731

    Plasmid
    #142201
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PROX1 (PROX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142201-plasmid-map-sequence-id-397649

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  5. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Article
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    75143-plasmid-map-sequence-id-147264

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  6. hKv2.1-pEGFP-C1

    Plasmid
    #111538
    Purpose
    Expresses human KCNB1 in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human Kv2.1 (KCNB1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111538-plasmid-map-sequence-id-217177

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  11. pLV-Blast_hCOL4A5

    Plasmid
    #198100
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198100-plasmid-map-sequence-id-389263

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  16. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  20. FLAG-hPAX3 (#395)

    Plasmid
    #78335
    Purpose
    Overexpression of human PAX3 ORF with a N-terminal FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    PAX3 (PAX3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 3641 - 3660 of 169450 results