167,913 results

1. pDONR P2R-P3-EGFP

   Plasmid

   #48349

   Purpose

   Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Mutation

   Aminoacid 40 is valine in our sequence, while it …

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE0029

   Plasmid

   #120730

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Type

   Empty backbone

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCURE

   Plasmid

   #23013

   Depositor

   James Bowie

   Article

   Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

   Insert

   I-CreI homing endonuclease
   

   Expression

   Bacterial

   Mutation

   I-CreI expression is controlled by the arabinose …

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMEL13

   Plasmid

   #107919

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-NES-jRCaMP1b

   Plasmid

   #63136

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1b
   

   Tags

   nuclear export signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDESTmycQKI

   Plasmid

   #19870

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   quaking homolog, KH domain RNA binding (QKI Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

7. CVS-N2c-nl.EGFP-jRGECO1a

   Plasmid

   #172386

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + jRGECO1a
   

   Use

   Neurotropic virus

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. tdTomato-Lysosomes-20

   Plasmid

   #58109

   Purpose

   Localization: Lysosome Membrane, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSLQ-mgU2-47

   Plasmid

   #127585

   Purpose

   Expresses mgU2-47 scaRNA from a mU6 promoter

   Depositor

   David Rueda

   Article

   Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

   Insert

   mgU2-47 scaRNA
   

   Expression

   Mammalian

   Promoter

   modified U6

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM37443

    Plasmid

    #153218

    Purpose

    Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS426 TEF mCherry

    Plasmid

    #129433

    Purpose

    express mcherry

    Depositor

    Oskar Laur

    Insert

    mcherry
    

    Expression

    Yeast

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-FLEX-jYCaMP1s

    Plasmid

    #135420

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEL12

    Plasmid

    #107918

    Purpose

    hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-IRES-GFP II (pMIG II)

    Plasmid

    #52107

    Purpose

    a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-SPN_ARR wt (Shank3)

    Plasmid

    #175254

    Purpose

    SPN-ARR fragment in EGFP backbone for mammalian expression

    Depositor

    Johanna Ivaska

    Article

    Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

    Insert

    SPN-ARR fragment from SHANK3
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-P-RAN-H2A2B

    Plasmid

    #106412

    Purpose

    P-RAN-H2A2B (RANbody H2A2B::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-H2A2B
    

    Tags

    HA and His

    Expression

    Mammalian

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. RFP-eNOS-NLS

    Plasmid

    #22496

    Depositor

    William Sessa

    Article

    Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

    Insert

    eNOS(G2A)-NLS
    

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

19. YCPlac33-sGFP-VRG4

    Plasmid

    #25447

    Depositor

    Benjamin Glick

    Article

    Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

    Insert

    Synthetic Green Fluorescent Protein - VRG4
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Expression

    Mammalian

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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