PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49425||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4898
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameautophagy-related 8
SpeciesS. cerevisiae (budding yeast)
Entrez GeneATG8 (a.k.a. YBL078C, APG8, AUT7, CVT5)
- Promoter ATG8
/ Fusion Protein
- GFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFP-ATG8(416)/GFP-AUT7(416) was a gift from Daniel Klionsky (Addgene plasmid # 49425 ; http://n2t.net/addgene:49425 ; RRID:Addgene_49425)
For your References section:Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris. Guan J, Stromhaug PE, George MD, Habibzadegah-Tari P, Bevan A, Dunn WA Jr, Klionsky DJ. Mol Biol Cell. 2001 Dec;12(12):3821-38. 10.1091/mbc.12.12.3821 PubMed 11739783