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Addgene

CuGFP-ATG8(416)
(Plasmid #49423)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49423 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS416
  • Backbone size w/o insert (bp) 4898
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    autophagy-related 8
  • Alt name
    ATG8
  • Alt name
    AUT7
  • Alt name
    APG8
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ATG8 (a.k.a. YBL078C, APG8, AUT7, CVT5)
  • Promoter CUP1
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CuGFP-ATG8(416) was a gift from Daniel Klionsky (Addgene plasmid # 49423 ; http://n2t.net/addgene:49423 ; RRID:Addgene_49423)
  • For your References section:

    Phosphatidylinositol 4-kinases are required for autophagic membrane trafficking. Wang K, Yang Z, Liu X, Mao K, Nair U, Klionsky DJ. J Biol Chem. 2012 Nov 2;287(45):37964-72. doi: 10.1074/jbc.M112.371591. Epub 2012 Sep 13. 10.1074/jbc.M112.371591 PubMed 22977244