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pDCC6
(Plasmid #59985)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59985 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Alt name
    3xFLAG-hSpCas9
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    4266
  • Promoter hsp70Bb
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    U6-2>sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    501
  • Promoter U6-96Ab

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDCC6 was a gift from Peter Duchek (Addgene plasmid # 59985 ; http://n2t.net/addgene:59985 ; RRID:Addgene_59985)
  • For your References section:

    Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila. Gokcezade J, Sienski G, Duchek P. G3 (Bethesda). 2014 Sep 17. pii: g3.114.014126. doi: 10.1534/g3.114.014126. 10.1534/g3.114.014126 PubMed 25236734