PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62209||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturergift from Silvia Aldaz
- Backbone size w/o insert (bp) 12429
- Total vector size (bp) 16571
Vector typeInsect Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Promoter act5C
- Cloning method Gibson Cloning
- 5′ sequencing primer GGTAGACCAGCGCAGTCCAAGG
- 3′ sequencing primer TAGAGCTTTAAATCTCTGTAGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAct-Cas9 was a gift from Simon Bullock (Addgene plasmid # 62209 ; http://n2t.net/addgene:62209 ; RRID:Addgene_62209)
For your References section:Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Port F, Chen HM, Lee T, Bullock SL. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. 10.1073/pnas.1405500111 PubMed 25002478