Purposemultiplex gRNA plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73914||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerNorbert Perrimon, Jian-Quan Ni, Harvard
- Total vector size (bp) 6248
Vector typeInsect Expression, CRISPR
Growth in Bacteria
SpeciesD. melanogaster (fly)
Insert Size (bp)750
- Promoter dU6:3
- Cloning method Gibson Cloning
- 5′ sequencing primer ACGTTTTATAACTTATGCCCCTAAG
- 3′ sequencing primer GCCGAGCACAATTGTCTAGAATGC (Common Sequencing Primers)
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFD5 was a gift from Simon Bullock (Addgene plasmid # 73914 ; http://n2t.net/addgene:73914 ; RRID:Addgene_73914)
For your References section:Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Port F, Bullock SL. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. 10.1038/nmeth.3972 PubMed 27595403