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pCFD7
(Plasmid #73916)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73916 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pValium22
  • Backbone manufacturer
    Norbert Perrimon, Jian-Quan Ni, Harvard
  • Total vector size (bp) 6248
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tRNA:hAsCpf1-gRNA:tRNA
  • gRNA/shRNA sequence
    empty
  • Species
    H. sapiens (human), D. melanogaster (fly)
  • Promoter dU6:3

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACGTTTTATAACTTATGCCCCTAAG
  • 3′ sequencing primer GCCGAGCACAATTGTCTAGAATGC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.

Visit crisprflydesign.org for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFD7 was a gift from Simon Bullock (Addgene plasmid # 73916 ; http://n2t.net/addgene:73916 ; RRID:Addgene_73916)
  • For your References section:

    Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Port F, Bullock SL. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. 10.1038/nmeth.3972 PubMed 27595403