|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62210||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerJohannes Bischof and Konrad Basler
- Backbone size w/o insert (bp) 9200
- Total vector size (bp) 13981
Vector typeInsect Expression
Growth in Bacteria
Insert Size (bp)4770
- Promoter nanos
- Cloning method Gibson Cloning
- 5′ sequencing primer AGTATCGCGAATACTTCAGTTG
- 3′ sequencing primer CTGGTTAACCCAGCTTTGATCG (Common Sequencing Primers)
Terms and Licenses
Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pnos-Fok1:dCas9-nos was a gift from Simon Bullock (Addgene plasmid # 62210 ; http://n2t.net/addgene:62210 ; RRID:Addgene_62210)