|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62211||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturergift from Silvia Aldaz
- Backbone size w/o insert (bp) 12429
- Total vector size (bp) 17201
Vector typeInsect Expression
Growth in Bacteria
Insert Size (bp)4770
- Promoter act5C
- Cloning method Gibson Cloning
- 5′ sequencing primer GCGCAGTCCAAGGAAACCACGC
- 3′ sequencing primer TGTCCAATTATGTCACACCACAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAct-Fok1:dCas9 was a gift from Simon Bullock (Addgene plasmid # 62211 ; http://n2t.net/addgene:62211 ; RRID:Addgene_62211)