PurposeExpresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62208||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerJohannes Bischof and Konrad Basler
- Backbone size w/o insert (bp) 9200
- Total vector size (bp) 13353
Vector typeInsect Expression
Growth in Bacteria
- Promoter nanos
- Cloning method Gibson Cloning
- 5′ sequencing primer GATAAAGAAGTATCGCGAATAC
- 3′ sequencing primer TTACTATCTATCTGGTTAACCC (Common Sequencing Primers)
Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pnos-Cas9-nos was a gift from Simon Bullock (Addgene plasmid # 62208 ; http://n2t.net/addgene:62208 ; RRID:Addgene_62208)
For your References section:Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Port F, Chen HM, Lee T, Bullock SL. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. 10.1073/pnas.1405500111 PubMed 25002478