Purposeexpression of gRNA under control of the Drosophila U6:3 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49410||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerNorbert Perrimon, Jian-Quan Ni, Harvard
- Total vector size (bp) 6248
Vector typeInsect Expression, CRISPR
Growth in Bacteria
SpeciesD. melanogaster (fly)
Insert Size (bp)750
- Promoter dU6-3
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ACCTACTCAGCCAAGAGGC
- 3′ sequencing primer TGCATACGCATTAAGCGAAC (Common Sequencing Primers)
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.
Visit crisprflydesign.org for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFD3-dU6:3gRNA was a gift from Simon Bullock (Addgene plasmid # 49410 ; http://n2t.net/addgene:49410 ; RRID:Addgene_49410)
For your References section:Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Port F, Chen HM, Lee T, Bullock SL. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. 10.1073/pnas.1405500111 PubMed 25002478