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pCFD3-dU6:3gRNA Citations (48)

Plasmid Article: Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.
Port F, Chen HM, Lee T, Bullock SL Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
PubMed Journal

Articles Citing pCFD3-dU6:3gRNA

Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations. Gantz VM, Bier E. Science. 2015 Apr 24;348(6233):442-4. doi: 10.1126/science.aaa5945. Epub 2015 Mar 19. PubMed
Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research. Port F, Muschalik N, Bullock SL. G3 (Bethesda). 2015 May 20;5(7):1493-502. doi: 10.1534/g3.115.019083. PubMed
A CRISPR/Cas9 mediated point mutation in the alpha 6 subunit of the nicotinic acetylcholine receptor confers resistance to spinosad in Drosophila melanogaster. Zimmer CT, Garrood WT, Puinean AM, Eckel-Zimmer M, Williamson MS, Davies TG, Bass C. Insect Biochem Mol Biol. 2016 Jun;73:62-9. doi: 10.1016/j.ibmb.2016.04.007. Epub 2016 Apr 24. PubMed
Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9. Lamb AM, Walker EA, Wittkopp PJ. Fly (Austin). 2016 Aug 5:1-12. PubMed

Associated Plasmids

Creating Heritable Mutations in Drosophila with CRISPR-Cas9. Port F, Bullock SL. Methods Mol Biol. 2016;1478:145-160. PubMed
Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy. Kane NS, Vora M, Varre KJ, Padgett RW. G3 (Bethesda). 2017 Jan 5;7(1):87-93. doi: 10.1534/g3.116.036723. PubMed

Associated Plasmids

Using CRISPR-Cas9 to Study ERK Signaling in Drosophila. Fores M, Papagianni A, Rodriguez-Munoz L, Jimenez G. Methods Mol Biol. 2017;1487:353-365. PubMed
Probing the canonicity of the Wnt/Wingless signaling pathway. Franz A, Shlyueva D, Brunner E, Stark A, Basler K. PLoS Genet. 2017 Apr 3;13(4):e1006700. doi: 10.1371/journal.pgen.1006700. eCollection 2017 Apr. PubMed
MCTP is an ER-resident calcium sensor that stabilizes synaptic transmission and homeostatic plasticity. Genc O, Dickman DK, Ma W, Tong A, Fetter RD, Davis GW. Elife. 2017 May 9;6. pii: e22904. doi: 10.7554/eLife.22904. PubMed
Patterned cortical tension mediated by N-cadherin controls cell geometric order in the Drosophila eye. Chan EH, Chavadimane Shivakumar P, Clement R, Laugier E, Lenne PF. Elife. 2017 May 24;6. pii: e22796. doi: 10.7554/eLife.22796. PubMed
Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations. Champer J, Reeves R, Oh SY, Liu C, Liu J, Clark AG, Messer PW. PLoS Genet. 2017 Jul 20;13(7):e1006796. doi: 10.1371/journal.pgen.1006796. eCollection 2017 Jul. PubMed
Increased acetylation of microtubules rescues human tau-induced microtubule defects and neuromuscular junction abnormalities in Drosophila. Mao CX, Wen X, Jin S, Zhang YQ. Dis Model Mech. 2017 Oct 1;10(10):1245-1252. doi: 10.1242/dmm.028316. Epub 2017 Aug 17. PubMed
CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity. Xu XS, Gantz VM, Siomava N, Bier E. Elife. 2017 Dec 23;6. pii: 30281. doi: 10.7554/eLife.30281. PubMed
A postsynaptic PI3K-cII dependent signaling controller for presynaptic homeostatic plasticity. Hauswirth AG, Ford KJ, Wang T, Fetter RD, Tong A, Davis GW. Elife. 2018 Jan 5;7. pii: 31535. doi: 10.7554/eLife.31535. PubMed
The bHLH Protein Nulp1 is Essential for Femur Development Via Acting as a Cofactor in Wnt Signaling in Drosophila. Zeng Q, Wan Y, Zhu P, Zhao M, Jiang F, Chen J, Tang M, Zhu X, Li Y, Zha H, Wang Y, Hu M, Mo X, Zhang Y, Chen Y, Chen Y, Ye X, Bodmer R, Ocorr K, Jiang Z, Zhuang J, Yuan W, Wu X. Curr Mol Med. 2018 Mar 9;17(7):509-517. doi: 10.2174/1566524018666180212145714. PubMed
Mechanical strain regulates the Hippo pathway in Drosophila. Fletcher GC, Diaz-de-la-Loza MD, Borreguero-Munoz N, Holder M, Aguilar-Aragon M, Thompson BJ. Development. 2018 Mar 8;145(5). pii: dev.159467. doi: 10.1242/dev.159467. PubMed
Visualization of Transvection in Living Drosophila Embryos. Lim B, Heist T, Levine M, Fukaya T. Mol Cell. 2018 Apr 19;70(2):287-296.e6. doi: 10.1016/j.molcel.2018.02.029. Epub 2018 Mar 29. PubMed
Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Tan KL, Haelterman NA, Kwartler CS, Regalado ES, Lee PT, Nagarkar-Jaiswal S, Guo DC, Duraine L, Wangler MF, Bamshad MJ, Nickerson DA, Lin G, Milewicz DM, Bellen HJ. Dev Cell. 2018 Apr 23;45(2):226-244.e8. doi: 10.1016/j.devcel.2018.03.020. PubMed
Reducing resistance allele formation in CRISPR gene drive. Champer J, Liu J, Oh SY, Reeves R, Luthra A, Oakes N, Clark AG, Messer PW. Proc Natl Acad Sci U S A. 2018 May 22;115(21):5522-5527. doi: 10.1073/pnas.1720354115. Epub 2018 May 7. PubMed
The Drosophila homologue of MEGF8 is essential for early development. Lloyd DL, Toegel M, Fulga TA, Wilkie AOM. Sci Rep. 2018 Jun 8;8(1):8790. doi: 10.1038/s41598-018-27076-y. PubMed
Gorab is a Golgi protein required for structure and duplication of Drosophila centrioles. Kovacs L, Chao-Chu J, Schneider S, Gottardo M, Tzolovsky G, Dzhindzhev NS, Riparbelli MG, Callaini G, Glover DM. Nat Genet. 2018 Jul;50(7):1021-1031. doi: 10.1038/s41588-018-0149-1. Epub 2018 Jun 11. PubMed
gamma-TuRC Heterogeneity Revealed by Analysis of Mozart1. Tovey CA, Tubman CE, Hamrud E, Zhu Z, Dyas AE, Butterfield AN, Fyfe A, Johnson E, Conduit PT. Curr Biol. 2018 Jul 23;28(14):2314-2323.e6. doi: 10.1016/j.cub.2018.05.044. Epub 2018 Jul 5. PubMed
An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. Li-Kroeger D, Kanca O, Lee PT, Cowan S, Lee MT, Jaiswal M, Salazar JL, He Y, Zuo Z, Bellen HJ. Elife. 2018 Aug 9;7. pii: 38709. doi: 10.7554/eLife.38709. PubMed
Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs. Oberhofer G, Ivy T, Hay BA. Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):E9343-E9352. doi: 10.1073/pnas.1805278115. Epub 2018 Sep 17. PubMed
Molecular Interface of Neuronal Innate Immunity, Synaptic Vesicle Stabilization, and Presynaptic Homeostatic Plasticity. Harris N, Fetter RD, Brasier DJ, Tong A, Davis GW. Neuron. 2018 Dec 5;100(5):1163-1179.e4. doi: 10.1016/j.neuron.2018.09.048. Epub 2018 Oct 18. PubMed
Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1. Glenn SE, Geyer PK. G3 (Bethesda). 2019 Feb 7;9(2):345-357. doi: 10.1534/g3.118.200705. PubMed
Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos. Mir M, Stadler MR, Ortiz SA, Hannon CE, Harrison MM, Darzacq X, Eisen MB. Elife. 2018 Dec 27;7. pii: 40497. doi: 10.7554/eLife.40497. PubMed
Molecular safeguarding of CRISPR gene drive experiments. Champer J, Chung J, Lee YL, Liu C, Yang E, Wen Z, Clark AG, Messer PW. Elife. 2019 Jan 22;8. pii: 41439. doi: 10.7554/eLife.41439. PubMed
Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive. Oberhofer G, Ivy T, Hay BA. Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):6250-6259. doi: 10.1073/pnas.1816928116. Epub 2019 Feb 13. PubMed
Axin proteolysis by Iduna is required for the regulation of stem cell proliferation and intestinal homeostasis in Drosophila. Gultekin Y, Steller H. Development. 2019 Mar 15;146(6). pii: dev.169284. doi: 10.1242/dev.169284. PubMed
Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila. Meltzer H, Marom E, Alyagor I, Mayseless O, Berkun V, Segal-Gilboa N, Unger T, Luginbuhl D, Schuldiner O. Nat Commun. 2019 May 8;10(1):2113. doi: 10.1038/s41467-019-10140-0. PubMed
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling. Shami Shah A, Batrouni AG, Kim D, Punyala A, Cao W, Han C, Goldberg ML, Smolka MB, Baskin JM. Cell Rep. 2019 May 14;27(7):2157-2170.e8. doi: 10.1016/j.celrep.2019.04.060. PubMed
Strip and Cka negatively regulate JNK signalling during Drosophila spermatogenesis. La Marca JE, Diepstraten ST, Hodge AL, Wang H, Hart AH, Richardson HE, Somers WG. Development. 2019 Jun 28;146(13). pii: dev.174292. doi: 10.1242/dev.174292. PubMed
Parental allele-specific protein expression in single cells In vivo. Lo CA, Chen BE. Dev Biol. 2019 Jun 11. pii: S0012-1606(19)30221-0. doi: 10.1016/j.ydbio.2019.06.004. PubMed
piRNA-guided co-transcriptional silencing coopts nuclear export factors. Fabry MH, Ciabrelli F, Munafo M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B. Elife. 2019 Jun 20;8. pii: 47999. doi: 10.7554/eLife.47999. PubMed
PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development. Liu K, Jones S, Minis A, Rodriguez J, Molina H, Steller H. Dev Cell. 2019 Aug 19;50(4):509-524.e10. doi: 10.1016/j.devcel.2019.06.009. Epub 2019 Jul 18. PubMed
Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation. Fulford AD, Holder MV, Frith D, Snijders AP, Tapon N, Ribeiro PS. Elife. 2019 Sep 30;8. pii: 46592. doi: 10.7554/eLife.46592. PubMed
F654A and K558Q Mutations in NMDA Receptor 1 Affect Ethanol-Induced Behaviors in Drosophila. Troutwine B, Park A, Velez-Hernandez ME, Lew L, Mihic SJ, Atkinson NS. Alcohol Clin Exp Res. 2019 Dec;43(12):2480-2493. doi: 10.1111/acer.14215. Epub 2019 Nov 1. PubMed
The Hippo pathway integrates PI3K-Akt signals with mechanical and polarity cues to control tissue growth. Borreguero-Munoz N, Fletcher GC, Aguilar-Aragon M, Elbediwy A, Vincent-Mistiaen ZI, Thompson BJ. PLoS Biol. 2019 Oct 15;17(10):e3000509. doi: 10.1371/journal.pbio.3000509. eCollection 2019 Oct. PubMed
Peptidoglycan-dependent NF-kappaB activation in a small subset of brain octopaminergic neurons controls female oviposition. Masuzzo A, Maniere G, Viallat-Lieutaud A, Avazeri E, Zugasti O, Grosjean Y, Kurz CL, Royet J. Elife. 2019 Oct 29;8. pii: 50559. doi: 10.7554/eLife.50559. PubMed
Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEET. Huynh N, Ou Q, Cox P, Lill R, King-Jones K. Nat Commun. 2019 Nov 29;10(1):5463. doi: 10.1038/s41467-019-13237-8. PubMed
Dopamine modulates the optomotor response to unreliable visual stimuli in Drosophila melanogaster. Akiba M, Sugimoto K, Aoki R, Murakami R, Miyashita T, Hashimoto R, Hiranuma A, Yamauchi J, Ueno T, Morimoto T. Eur J Neurosci. 2020 Feb;51(3):822-839. doi: 10.1111/ejn.14648. Epub 2019 Dec 30. PubMed
Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes. Bosch JA, Knight S, Kanca O, Zirin J, Yang-Zhou D, Hu Y, Rodiger J, Amador G, Bellen HJ, Perrimon N, Mohr SE. Curr Protoc Mol Biol. 2020 Mar;130(1):e112. doi: 10.1002/cpmb.112. PubMed
Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time. Sidor C, Stevens TJ, Jin L, Boulanger J, Roper K. Dev Cell. 2020 Feb 10;52(3):364-378.e7. doi: 10.1016/j.devcel.2019.12.003. Epub 2020 Jan 2. PubMed
Tissue-Scale Mechanical Coupling Reduces Morphogenetic Noise to Ensure Precision during Epithelial Folding. Eritano AS, Bromley CL, Bolea Albero A, Schutz L, Wen FL, Takeda M, Fukaya T, Sami MM, Shibata T, Lemke S, Wang YC. Dev Cell. 2020 Apr 20;53(2):212-228.e12. doi: 10.1016/j.devcel.2020.02.012. Epub 2020 Mar 12. PubMed
Nucleolar stress in Drosophila neuroblasts, a model for human ribosomopathies. Baral SS, Lieux ME, DiMario PJ. Biol Open. 2020 Apr 13;9(4). pii: bio.046565. doi: 10.1242/bio.046565. PubMed
Intimate functional interactions between TGS1 and the Smn complex revealed by an analysis of the Drosophila eye development. Maccallini P, Bavasso F, Scatolini L, Bucciarelli E, Noviello G, Lisi V, Palumbo V, D'Angeli S, Cacchione S, Cenci G, Ciapponi L, Wakefield JG, Gatti M, Raffa GD. PLoS Genet. 2020 May 26;16(5):e1008815. doi: 10.1371/journal.pgen.1008815. eCollection 2020 May. PubMed
Tissue specific requirement of Drosophila Rcd4 for centriole duplication and ciliogenesis. Panda P, Kovacs L, Dzhindzhev N, Fatalska A, Persico V, Geymonat M, Riparbelli MG, Callaini G, Glover DM. J Cell Biol. 2020 Sep 7;219(8). pii: 151861. doi: 10.1083/jcb.201912154. PubMed

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