Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9.
Lamb AM, Walker EA, Wittkopp PJ
Fly (Austin). 2016 Aug 5:1-12. PubMed Article
Lamb AM, Walker EA, Wittkopp PJ
Fly (Austin). 2016 Aug 5:1-12. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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83473 | pGEM-WingGFP-tan | EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice. |
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83474 | pGEM-3XP3.RFP-tan | RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice. |
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