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Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9.
Lamb AM, Walker EA, Wittkopp PJ
Fly (Austin). 2016 Aug 5:1-12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
83473pGEM-WingGFP-tanEGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
83474pGEM-3XP3.RFP-tanRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

Antibodies from Article