|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171568||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAh-Ram Kim (Perrimon Lab)
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneCXCR2 (a.k.a. CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB)
/ Fusion Proteins
- PelB signal peptide (N terminal on insert)
- ALFA-tag/His-tag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
A derivative of pET-26b (pET-26b-Nb-GGA, BsaI-Golden Gate Assembly vector) was used to clone this vector.
Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-26b-Nb127D01-ALFA-His was a gift from Norbert Perrimon & Hidde Ploegh (Addgene plasmid # 171568 ; http://n2t.net/addgene:171568 ; RRID:Addgene_171568)
For your References section:Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies. Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, Ploegh H, Perrimon N. bioRxiv 2021.04.16.440240 10.1101/2021.04.16.440240