Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET-26b-Nb127D01-ALFA-His
(Plasmid #171568)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 171568 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-26b-Nb-GGA
  • Backbone manufacturer
    Ah-Ram Kim (Perrimon Lab)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nb127D01-ALFA-His
  • Species
    H. sapiens (human)
  • Entrez Gene
    CXCR2 (a.k.a. CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB)
  • Tags / Fusion Proteins
    • PelB signal peptide (N terminal on insert)
    • ALFA-tag/His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

A derivative of pET-26b (pET-26b-Nb-GGA, BsaI-Golden Gate Assembly vector) was used to clone this vector.

Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-26b-Nb127D01-ALFA-His was a gift from Norbert Perrimon & Hidde Ploegh (Addgene plasmid # 171568 ; http://n2t.net/addgene:171568 ; RRID:Addgene_171568)
  • For your References section:

    Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies. Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, Ploegh H, Perrimon N. bioRxiv 2021.04.16.440240 10.1101/2021.04.16.440240