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Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies.
Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, Ploegh HL, Perrimon N
Elife. 2022 Jan 25;11. pii: 74326. doi: 10.7554/eLife.74326.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
171564pMT-HGP-v3-Nb127D01-hIgGExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells
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171565pMT-HGP-v3-NbVHH05-hIgGExpresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells
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171566pET-26b-Nb127D01-HA-HisBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)
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171567pET-26b-NbVHH05-HA-HisBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)
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171568pET-26b-Nb127D01-ALFA-HisBacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)
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171569pET-26b-NbVHH05-ALFA-HisBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)
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171570pAW-NbVHH05-GFPExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells
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171571pAW-Nb127D01-GFPExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells
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171572pAW-NbVHH05-mCherryExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells
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171573pAW-Nb127D01-mCherryExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells
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171574pAW-BiP-NbVHH05-mCherry-KDELExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane
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171575pAW-BiP-Nb127D01-mCherry-KDELExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane
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171576pAW-CD8-NbVHH05-GFPExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane
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171577pAW-CD8-Nb127D01-GFPExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane
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171578pScarlessHD-C-3x127D01-DsRedFor scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
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171579pScarlessHD-N-3x127D01-DsRedFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
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171580pScarlessHD-C-3xVHH05-DsRedFor scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
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171581pScarlessHD-N-3xVHH05-DsRedFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
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Antibodies from Article