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Precise genome engineering in Drosophila using prime editing.
Bosch JA, Birchak G, Perrimon N
Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 10.1073/pnas.2021996118.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
149545pCFD3-NSExpression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
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149546pCFD5-NSExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
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149548pEntr_PE2Gateway entry clone with PE2 enzyme (with stop codon)
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149549pNos-PE2-attBExpression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.
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149550pUAS-PE2-attBExpression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.
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149552pAct-PE2-HygroRExpression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.
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149610pAct-GW-HygroRGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.
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Antibodies from Article