pDsRed-attP
(Plasmid #51019)

• Purpose: Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
• Depositing Labs: Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
• Publication: CRISPR dsDNA donor vectors (unpublished)
• Sequence Information:

  • Depositor Sequences: Full (1)
  • Addgene Sequences: Partial (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	51019	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pJ204
  (Search Vector Database)
• Backbone manufacturer: DNA 2.0
• Backbone size w/o insert (bp): 2703
• Total vector size (bp): 4098
• Vector type: high copy, amp resistance

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: attP
• Species: T4 phage
• Insert Size (bp): 50
• Promoter: None

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Synthesized, not cloned
• 3′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: LoxP-3xP3-DsRed-LoxP
• Species: Synthetic
• Insert Size (bp): 1225
• Promoter: 3xP3

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Synthesized, not cloned
• 3′ sequencing primer: NA

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: We had it synthesized by DNA2.0.
• Terms and Licenses:
  • UBMTA
  • Clontech Limited Use Label License
• Articles Citing this Plasmid:
  • 9 References

Depositor Comments

This plasmid is also known as pHD-DsRed-attP (referenced in publication).