pDsRed-attP
(Plasmid #51019)

• Purpose: Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
• Depositing Labs: Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
• Publication: Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. Epub 2014 Jan 29.

Backbone

• Vector backbone: pJ204
  (Search Vector Database)
• Backbone manufacturer: DNA 2.0
• Backbone size w/o insert (bp): 2703
• Total vector size (bp): 4098
• Vector type: high copy, amp resistance

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: attP
• Species: T4 phage
• Insert Size (bp): 50
• Promoter: None

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Synthesized, not cloned
• 3′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: LoxP-3xP3-DsRed-LoxP
• Species: Synthetic
• Insert Size (bp): 1225
• Promoter: 3xP3

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Synthesized, not cloned
• 3′ sequencing primer: NA

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: We had it synthesized by DNA2.0.
• Articles Citing this Plasmid:
  • 42 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is also known as pHD-DsRed-attP (referenced in publication).