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PurposeVector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 51019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJ204
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Backbone manufacturerDNA 2.0
- Backbone size w/o insert (bp) 2703
- Total vector size (bp) 4098
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Vector typehigh copy, amp resistance
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameattP
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SpeciesT4 phage
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Insert Size (bp)50
- Promoter None
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer Synthesized, not cloned
- 3′ sequencing primer NA (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameLoxP-3xP3-DsRed-LoxP
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SpeciesSynthetic
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Insert Size (bp)1225
- Promoter 3xP3
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer Synthesized, not cloned
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byWe had it synthesized by DNA2.0.
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
This plasmid is also known as pHD-DsRed-attP (referenced in publication).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDsRed-attP was a gift from Melissa Harrison & Kate O'Connor-Giles & Jill Wildonger (Addgene plasmid # 51019 ; http://n2t.net/addgene:51019 ; RRID:Addgene_51019)