PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46294||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2958
Vector typeInsect Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namecodon optimized Cas9
MutationSilent change from C to A at bp 834 of insert
- Promoter Hsp70
/ Fusion Protein
- 3x Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byCas9 insert from Feng Zhang, Broad Institute, MIT
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid was created by subcloning the Hsp70-Cas9 containing NotI fragment from Addgene plasmid 45945 in NotI digested pBluescript-KS(+).
For more information on FlyCRISPR Plasmids please refer to: http://www.addgene.org/crispr/OConnor-Giles/
Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-Hsp70-Cas9 was a gift from Melissa Harrison & Kate O'Connor-Giles & Jill Wildonger (Addgene plasmid # 46294 ; http://n2t.net/addgene:46294 ; RRID:Addgene_46294)
For your References section:Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila. Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings AM, O'Connor-Giles KM. Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. Epub 2014 Jan 29. 10.1534/genetics.113.160713 PubMed 24478335