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Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila.
Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings AM, O'Connor-Giles KM
Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. Epub 2014 Jan 29. PubMed Article

Plasmids from Article

ID Plasmid Purpose
46294pBS-Hsp70-Cas9A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
51019pDsRed-attPVector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
51434pHD-DsRedVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

Antibodies from Article