PurposeVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51434||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDNA 2.0
- Backbone size w/o insert (bp) 2703
- Total vector size (bp) 4048
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)1225
- Promoter 3xP3
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byWe had it synthesized by DNA2.0.
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHD-DsRed was a gift from Kate O'Connor-Giles (Addgene plasmid # 51434 ; http://n2t.net/addgene:51434 ; RRID:Addgene_51434)
For your References section:Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila. Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings AM, O'Connor-Giles KM. Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. Epub 2014 Jan 29. 10.1534/genetics.113.160713 PubMed 24478335