Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pBS-Hsp70-Cas9 Citations (26)

Originally described in: Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila.
Gratz SJ, Ukken FP, Rubinstein CD, Thiede G, Donohue LK, Cummings AM, O'Connor-Giles KM Genetics. 2014 Apr;196(4):961-71. doi: 10.1534/genetics.113.160713. Epub 2014 Jan 29.
PubMed Journal

Articles Citing pBS-Hsp70-Cas9

Easy quantitative assessment of genome editing by sequence trace decomposition. Brinkman EK, Chen T, Amendola M, van Steensel B. Nucleic Acids Res. 2014 Dec 16;42(22):e168. doi: 10.1093/nar/gku936. Epub 2014 Oct 9. PubMed
Cas9-based genome editing in Drosophila. Housden BE, Lin S, Perrimon N. Methods Enzymol. 2014;546:415-39. doi: 10.1016/B978-0-12-801185-0.00019-2. PubMed
Genome editing. The mutagenic chain reaction: a method for converting heterozygous to homozygous mutations. Gantz VM, Bier E. Science. 2015 Apr 24;348(6233):442-4. doi: 10.1126/science.aaa5945. Epub 2015 Mar 19. PubMed
Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9. Gratz SJ, Harrison MM, Wildonger J, O'Connor-Giles KM. Methods Mol Biol. 2015;1311:335-48. doi: 10.1007/978-1-4939-2687-9_22. PubMed
Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells. Sumiyoshi T, Sato K, Yamamoto H, Iwasaki YW, Siomi H, Siomi MC. Genes Dev. 2016 Jul 15;30(14):1617-22. doi: 10.1101/gad.283929.116. PubMed
Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9. Lamb AM, Walker EA, Wittkopp PJ. Fly (Austin). 2016 Aug 5:1-12. PubMed

Associated Plasmids

Use of the CRISPR-Cas9 system for genome editing in cultured Drosophila ovarian somatic cells. Ishizu H, Sumiyoshi T, Siomi MC. Methods. 2017 May 25. pii: S1046-2023(16)30326-7. doi: 10.1016/j.ymeth.2017.05.021. PubMed
Short-Form Bomanins Mediate Humoral Immunity in Drosophila. Lindsay SA, Lin SJH, Wasserman SA. J Innate Immun. 2018;10(4):306-314. doi: 10.1159/000489831. Epub 2018 Jun 19. PubMed
Temporal dynamics of pair-rule stripes in living Drosophila embryos. Lim B, Fukaya T, Heist T, Levine M. Proc Natl Acad Sci U S A. 2018 Aug 14;115(33):8376-8381. doi: 10.1073/pnas.1810430115. Epub 2018 Jul 30. PubMed
Modulating the Expression Strength of the Baculovirus/Insect Cell Expression System: A Toolbox Applied to the Human Tumor Suppressor SMARCB1/SNF5. Golas MM, Jayaprakash S, Le LTM, Zhao Z, Heras Huertas V, Jensen IS, Yuan J, Sander B. Mol Biotechnol. 2018 Nov;60(11):820-832. doi: 10.1007/s12033-018-0107-2. PubMed
Parental allele-specific protein expression in single cells In vivo. Lo CA, Chen BE. Dev Biol. 2019 Jun 11. pii: S0012-1606(19)30221-0. doi: 10.1016/j.ydbio.2019.06.004. PubMed
Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii. Ahmed HMM, Hildebrand L, Wimmer EA. BMC Biotechnol. 2019 Dec 5;19(1):85. doi: 10.1186/s12896-019-0588-5. PubMed
Bombardier Enables Delivery of Short-Form Bomanins in the Drosophila Toll Response. Lin SJH, Fulzele A, Cohen LB, Bennett EJ, Wasserman SA. Front Immunol. 2020 Jan 10;10:3040. doi: 10.3389/fimmu.2019.03040. eCollection 2019. PubMed
The Daisho Peptides Mediate Drosophila Defense Against a Subset of Filamentous Fungi. Cohen LB, Lindsay SA, Xu Y, Lin SJH, Wasserman SA. Front Immunol. 2020 Jan 23;11:9. doi: 10.3389/fimmu.2020.00009. eCollection 2020. PubMed
Piwi suppresses transcription of Brahma-dependent transposons via Maelstrom in ovarian somatic cells. Onishi R, Sato K, Murano K, Negishi L, Siomi H, Siomi MC. Sci Adv. 2020 Dec 11;6(50). pii: 6/50/eaaz7420. doi: 10.1126/sciadv.aaz7420. Print 2020 Dec. PubMed
A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling. Trivedi D, Cm V, Bisht K, Janardan V, Pandit A, Basak B, H S, Ramesh N, Raghu P. Elife. 2020 Dec 15;9. pii: 55793. doi: 10.7554/eLife.55793. PubMed
Functional Diversification, Redundancy, and Epistasis among Paralogs of the Drosophila melanogaster Obp50a-d Gene Cluster. Johnstun JA, Shankar V, Mokashi SS, Sunkara LT, Ihearahu UE, Lyman RL, Mackay TFC, Anholt RRH. Mol Biol Evol. 2021 May 4;38(5):2030-2044. doi: 10.1093/molbev/msab004. PubMed
CopyCatchers are versatile active genetic elements that detect and quantify inter-homolog somatic gene conversion. Li Z, Marcel N, Devkota S, Auradkar A, Hedrick SM, Gantz VM, Bier E. Nat Commun. 2021 May 11;12(1):2625. doi: 10.1038/s41467-021-22927-1. PubMed

Associated Plasmids

Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii. Yan Y, Kobayashi Y, Huang C, Liu B, Qian W, Wan F, Schetelig MF. Int J Mol Sci. 2021 Jun 23;22(13). pii: ijms22136724. doi: 10.3390/ijms22136724. PubMed
Cas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells. Miyata Y, Fuse H, Tokumoto S, Hiki Y, Deviatiiarov R, Yoshida Y, Yamada TG, Cornette R, Gusev O, Shagimardanova E, Funahashi A, Kikawada T. Sci Rep. 2021 Oct 5;11(1):19698. doi: 10.1038/s41598-021-98905-w. PubMed
A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells. Bai Y, Caussinus E, Leo S, Bosshardt F, Myachina F, Rot G, Robinson MD, Lehner CF. BMC Genomics. 2021 Oct 28;22(1):771. doi: 10.1186/s12864-021-08057-4. PubMed
Double-tap gene drive uses iterative genome targeting to help overcome resistance alleles. Bishop AL, Lopez Del Amo V, Okamoto EM, Bodai Z, Komor AC, Gantz VM. Nat Commun. 2022 May 9;13(1):2595. doi: 10.1038/s41467-022-29868-3. PubMed
The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters. Baumgartner L, Handler D, Platzer SW, Yu C, Duchek P, Brennecke J. Elife. 2022 Oct 4;11:e80067. doi: 10.7554/eLife.80067. PubMed
Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito Aedes aegypti via single-generation crosses and modeling. Reid W, Williams AE, Sanchez-Vargas I, Lin J, Juncu R, Olson KE, Franz AWE. G3 (Bethesda). 2022 Dec 1;12(12):jkac280. doi: 10.1093/g3journal/jkac280. PubMed
Dynamic interplay between non-coding enhancer transcription and gene activity in development. Hamamoto K, Umemura Y, Makino S, Fukaya T. Nat Commun. 2023 Feb 20;14(1):826. doi: 10.1038/s41467-023-36485-1. PubMed
Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR/Cas9. Hausjell CS, Klausberger M, Ernst W, Grabherr R. PLoS One. 2023 Jul 27;18(7):e0289178. doi: 10.1371/journal.pone.0289178. eCollection 2023. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.