Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish.
Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI
Development. 2011 Jan . 138(1):169-77.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 27320 | pENTR5'_ubi | Gateway entry vector containing 3.5 kb ubi promoter fragment, with L4 and R1 sequences for MultiSite Gateway cloning |
| 27321 | pENTR/D_creERt2 | Gateway entry vector containing Cre(ERTt), for 4-hydroxytamoxifen-controlled Cre recombinase activity |
| 27322 | pENTR5'_ubi:loxP-EGFP-loxP | Gateway entry vector containing ubi:loxP-EGFP-loxP. Used to assemble ubi:Switch construct. |
| 27323 | pDestTol2pA2_ubi:EGFP | MultiSite Gateway assembly vector, to generate transgenic zebrafish with ubiquitous expression of EGFP |