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pCMV-myc-LC3 (Atg8)
(Plasmid #24919)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24919 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-Myc
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LC3
  • Alt name
    Atg8
  • Alt name
    microtubule-associated protein 1 light chain 3
  • Species
    H. sapiens (human)
  • GenBank ID
    BC067797
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-Rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-myc-LC3 (Atg8) was a gift from Toren Finkel (Addgene plasmid # 24919 ; http://n2t.net/addgene:24919 ; RRID:Addgene_24919)
  • For your References section:

    A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy. Lee IH, Cao L, Mostoslavsky R, Lombard DB, Liu J, Bruns NE, Tsokos M, Alt FW, Finkel T. Proc Natl Acad Sci U S A. 2008 Mar 4. 105(9):3374-9. 10.1073/pnas.0712145105 PubMed 18296641