pcDNA3.1 puro Nodamura B2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17228||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 puro+
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNodamura B2
Insert Size (bp)429
Mutation2 silent point mutations
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
The gene was cloned into the KpnI/XbaI sites within the pcDNA3.1 puro+ vector. The pcDNA3.1 puro+ vector was derived from the pcDNA3.1 neo+ vector by swapping the puro cassette for the Neo gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 puro Nodamura B2 was a gift from Christopher Sullivan (Addgene plasmid # 17228 ; http://n2t.net/addgene:17228 ; RRID:Addgene_17228)
For your References section:A virus-encoded inhibitor that blocks RNA interference in mammalian cells. Sullivan CS, Ganem D. J Virol. 2005 Jun . 79(12):7371-9. 10.1128/JVI.79.12.7371-7379.2005 PubMed 15919892
Map uploaded by the depositor.