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pcDNA3.1 puro Nodamura B2
(Plasmid #17228)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 17228 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pcDNA3.1 puro+
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Nodamura B2
  • Species
    Nodamura Virus
  • Insert Size (bp)
  • Mutation
    2 silent point mutations
  • Entrez Gene

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The gene was cloned into the KpnI/XbaI sites within the pcDNA3.1 puro+ vector. The pcDNA3.1 puro+ vector was derived from the pcDNA3.1 neo+ vector by swapping the puro cassette for the Neo gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 puro Nodamura B2 was a gift from Christopher Sullivan (Addgene plasmid # 17228 ; ; RRID:Addgene_17228)
  • For your References section:

    A virus-encoded inhibitor that blocks RNA interference in mammalian cells. Sullivan CS, Ganem D. J Virol. 2005 Jun . 79(12):7371-9. 10.1128/JVI.79.12.7371-7379.2005 PubMed 15919892