pEGFP-C1 Nodamura B2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19716||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNodamura B2
Insert Size (bp)429
Mutation2 silent point mutations
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
The Nodamuravirus B2 gene is subcloned from the KpnI/ApaI fragment from pcDNA3.1+puro Nodamura B2 into pEGFP-C1 vector, making the EGFP fusion construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-C1 Nodamura B2 was a gift from Christopher Sullivan (Addgene plasmid # 19716 ; http://n2t.net/addgene:19716 ; RRID:Addgene_19716)
For your References section:A virus-encoded inhibitor that blocks RNA interference in mammalian cells. Sullivan CS, Ganem D. J Virol. 2005 Jun . 79(12):7371-9. 10.1128/JVI.79.12.7371-7379.2005 PubMed 15919892
Map uploaded by the depositor.