161,255 results

1. AAV-Syn-DIO-TRPV1-P2A-mCherry

   Plasmid

   #200831

   Purpose

   In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-mCherry (Trpv1 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-R2L3-IRES-puro-pA

   Plasmid

   #139529

   Purpose

   Provides IRES-puro-pA cassette on Gateway entry vector

   Depositor

   Minoru Ko

   Insert

   puromycin resistant gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIZ-Flag6His-Siwi

   Plasmid

   #50560

   Purpose

   Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

   Depositor

   Yukihide Tomari

   Article

   Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

   Insert

   Siwi (Aubergine )
   

   Use

   Tags

   6xHIS and FLAG

   Expression

   Insect

   Mutation

   Promoter

   OpIE2

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

   Plasmid

   #182353

   Purpose

   GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP217-Lenti-CMV-GFP-WPRE-pA

   Plasmid

   #62925

   Purpose

   pLenti vector backbone designed to express GFP from a CMV promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Green Fluorescent protein (GFP)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   1.3αCaMKII and CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM2-hPMCA4b

   Plasmid

   #47759

   Purpose

   mammalian expression of human PMCA4b

   Depositor

   John Penniston, Emanuel Strehler

   Article

   Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

   Insert

   PMCA4b (ATP2B4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-NLS-HA-B3

   Plasmid

   #51270

   Purpose

   Expression of the site-specific recombinase B3 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-B3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAND

   Plasmid

   #49377

   Purpose

   Expression of AND gate in bacteria

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   AND gate
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

   Plasmid

   #132962

   Purpose

   Firefly-luciferase single-strand annealing assay cloning backbone

   Depositor

   Gang Bao

   Article

   Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

   Insert

   Firefly Luciferase
   

   Use

   CRISPR and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Stop sequence between luciferase gene

   Promoter

   CMV

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-HsPeg10rc3

    Plasmid

    #174859

    Purpose

    Expresses recoded human Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Homo sapiens PEG10 (PEG10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBK.134

    Plasmid

    #187219

    Purpose

    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectively

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    A: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    A: pTet*; B: pBetI

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TET-PH-lox66FRT

    Plasmid

    #139561

    Purpose

    piggyBac expression vector with TET-ON system and puromycin selection

    Depositor

    Minoru Ko

    Insert

    PGK-puroR-pA cassette, inserted in PB-TET vector
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST_5xUAS:EB3-mScarlet-I

    Plasmid

    #192358

    Purpose

    EB3 tagged with mScarlet-I, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_GPHN.FingR-eGFP-CCR5TC

    Plasmid

    #46296

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR-eGFP-CCR5TC (GPHN Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-b3STOPb3-ZsGreen

    Plasmid

    #51266

    Purpose

    B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    b3STOPb3-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

    Plasmid

    #55642

    Purpose

    sCre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV; Scre-dependent

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-Emerald

    Plasmid

    #139549

    Purpose

    Emerald ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    Emerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-R2L3-IRES-BSD-pA

    Plasmid

    #139534

    Purpose

    Provides IRES-BSD-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    Blasticidin resistant gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-Con/Fon-mCherry (AAV8)

    Viral Prep

    #137132-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-mCherry (#137132). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-mCherry plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    mCherry (Cre and Flp-dependent)

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

20. Ii-Str_ManII-SBP-tagBFP

    Plasmid

    #65257

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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