165,671 results

1. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. H2B-GFP

   Plasmid

   #11680

   Depositor

   Geoff Wahl

   Article

   Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

   Insert

   Human histone H2B (H2BC11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-EF1A-Puro-hTBK1-EGFP

   Plasmid

   #221553

   Purpose

   Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Expotin 1

   Plasmid

   #42047

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Exportin-1 (XPO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFREE

   Plasmid

   #92050

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRhamBAD and ptet

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSK659

   Plasmid

   #205128

   Purpose

   Expression of GFP-SnxA in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   SnxA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a-FB-dCas9-puro

   Plasmid

   #100547

   Purpose

   Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Inserts

   N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
   puromycin
   

   Use

   CRISPR

   Tags

   FLAG, Biotin-acceptor-site (Avitag)

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. E-cadherin-GFP

    Plasmid

    #28009

    Depositor

    Jennifer Stow

    Article

    Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER20_mGFP

    Plasmid

    #190027

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVS104

    Plasmid

    #207393

    Purpose

    Conjugal helper plasmid

    Depositor

    Edward Ruby, Jason Peters, Eric Stabb

    Article

    Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVTNT-T7-KPNA2

    Plasmid

    #26678

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 2 (KPNA2 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti Lifeact-mRuby2 BlastR

    Plasmid

    #84384

    Purpose

    Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MP9956:SFG.GD3synthase-2A-GD2synthase

    Plasmid

    #75013

    Purpose

    Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

    Depositor

    Martin Pule

    Article

    Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

    Inserts

    murine GD3 synthase
    murine GD2 synthase
    

    Use

    Retroviral; Momlv retroviral vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPRv2 puro

    Plasmid

    #98290

    Purpose

    Variant of lentiCRISPRv2 that confers puromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4-UPRE-luc2P-Hygro

    Plasmid

    #101788

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xUPRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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