163,743 results

1. pBluescript II SK--POLQ

   Plasmid

   #64873

   Purpose

   cloning vector for human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_Gal4UAS_IRES_mC_pGK_tBFP

   Plasmid

   #79123

   Purpose

   Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINE

   Plasmid

   #26043

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His-lacZ-KHHHHHH
   

   Use

   Tags

   KHHHHHH and MAHHHHHHSSG

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK7WGF2::hCas9

   Plasmid

   #46965

   Purpose

   Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   hCas9
   

   Use

   CRISPR; Plant expression

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   35S

   Available since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-mCherry-N1

   Plasmid

   #31907

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. mcherry-DN KASH

   Plasmid

   #125553

   Purpose

   mcherry labeled DN-KASH

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Truncated form: only the KASH domain of nesprin-…

   Promoter

   CMV

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hCGNL1-Myc-HA

   Plasmid

   #236515

   Purpose

   For expression of hParacingulinL1 in mammalian cells

   Depositor

   Sandra Citi

   Article

   Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

   Insert

   CGNL1 (CGNL1 Human)
   

   Use

   Tags

   Myc-HA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Has Service

   AAV8

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMma PylT sfGPF 150TAG

   Plasmid

   #154766

   Purpose

   plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, U25C in PylT

   Promoter

   EF1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-hARFGAP2-HA

    Plasmid

    #206410

    Purpose

    Mammalian expression of hARFGAP2

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

    Insert

    ARFGAP2 (ARFGAP2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRPML1-HA

    Plasmid

    #18825

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST-SorLA-v2

    Plasmid

    #154900

    Purpose

    Expresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    SORL1 (SORL1 Human)
    

    Use

    Tags

    Venus fragment 2 (v2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDA019

    Plasmid

    #131452

    Purpose

    control expression vector

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFRT/TO/HIS/FLAG/HA-PRDX1

    Plasmid

    #38086

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PRDX1 peroxiredoxin 1 (PRDX1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6HisVDAC1/pCDNA3

    Plasmid

    #197320

    Purpose

    Plasmid expressing an antigen targeting the VDAC1 transporter

    Depositor

    Dan Minor

    Insert

    VDAC1 (VDAC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-HA-hTAF1

    Plasmid

    #17997

    Depositor

    Robert Tjian

    Article

    Ruppert et al Nature. 1993 Mar 11. 362(6416):175-9.

    Insert

    hTAF1 (TAF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCRISPomyces-2BD

    Plasmid

    #234429

    Purpose

    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified dCas9(D10A, H840A) with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS diaCas9_sgRNA

    Plasmid

    #74923

    Purpose

    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

    Depositor

    Per Winge

    Article

    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Cas9 module and sgRNA module

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. HR_G3BP1-V5-APEX2-GFP

    Plasmid

    #105284

    Purpose

    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
    

    Use

    Human homologous recombination donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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