|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14959||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1700
Entrez GeneSMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI / SalI (destroyed during cloning)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Site directed mutagenesis in the coding sequence of Tyr162 (from TAT to TAC) and Val163 (from GTG to GTT). The mutated sequence encodes the same amino acids. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA Flag-Smad4M was a gift from Joan Massague (Addgene plasmid # 14959 ; http://n2t.net/addgene:14959 ; RRID:Addgene_14959)
For your References section:Breast cancer bone metastasis mediated by the Smad tumor suppressor pathway. Kang Y, He W, Tulley S, Gupta GP, Serganova I, Chen CR, Manova-Todorova K, Blasberg R, Gerald WL, Massague J. Proc Natl Acad Sci U S A. 2005 Sep 27. 102(39):13909-14. 10.1073/pnas.0506517102 PubMed 16172383