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hCRISPRi-v2, genome-wide, top 5 sgRNAs/gene

Pooled library

#83969

Purpose

Version 2 of the human CRISPRi genome wide library

Depositor

Jonathan Weissman

Publication

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles
pMA3790

Plasmid

#70110

Purpose

mtDNA elimination/production of rho-0 cells

Depositor

Mikhail Alexeyev

Publication

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

EGFP
hUNG1 Y147A
Neo

Tags

None, human OTC mitochondrial targeting sequence,…

Expression

Mammalian

Mutation

Y147A, aa's 1-77 removed

Promoter

CMV, EF1a, and SV40 and native

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMA3790

Plasmid

#70110

Purpose

mtDNA elimination/production of rho-0 cells

Depositor

Mikhail Alexeyev

Publication

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

EGFP
hUNG1 Y147A
Neo

Tags

None, human OTC mitochondrial targeting sequence,…

Expression

Mammalian

Mutation

Y147A, aa's 1-77 removed

Promoter

CMV, EF1a, and SV40 and native

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLenti-myc-GLUT4-mCherry

Plasmid

#64049

Purpose

3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

Depositor

Weiping Han

Publication

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

GLUT4 (Slc2a4 Mouse)

Use

Lentiviral

Tags

c-Myc and mCherry

Expression

Mammalian

Promoter

Ubiquitin

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pLenti-myc-GLUT4-mCherry

Plasmid

#64049

Purpose

3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

Depositor

Weiping Han

Publication

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

GLUT4 (Slc2a4 Mouse)

Use

Lentiviral

Tags

c-Myc and mCherry

Expression

Mammalian

Promoter

Ubiquitin

Available since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pAVU6+27-F30-2xdBroccoli

Plasmid

#66842

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Publication

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Mammalian

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAVU6+27-F30-2xdBroccoli

Plasmid

#66842

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Publication

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Mammalian

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Publication

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tobacco etch virus (TEV) protease

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Publication

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tobacco etch virus (TEV) protease

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-P4M-SidMx2

Plasmid

#51472

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Publication

Hammond et al J Cell Biol. 2014 Apr 7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P4M-SidM(546-647)
P4M-SidM(546-647)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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GFP-P4M-SidMx2

Plasmid

#51472

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Publication

Hammond et al J Cell Biol. 2014 Apr 7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P4M-SidM(546-647)
P4M-SidM(546-647)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pTagRFP657-N1

Plasmid

#31959

Depositor

Vladislav Verkhusha

Publication

Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagRFP657

Expression

Mammalian

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pTagRFP657-N1

Plasmid

#31959

Depositor

Vladislav Verkhusha

Publication

Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagRFP657

Expression

Mammalian

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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mCherry-UtrCH

Plasmid

#26740

Depositor

William Bement

Publication

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Utrophin

Use

Reporter for f-actin

Tags

mCherry

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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mCherry-UtrCH

Plasmid

#26740

Depositor

William Bement

Publication

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Utrophin

Use

Reporter for f-actin

Tags

mCherry

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pNIC28-Bsa4

Plasmid

#26103

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pNIC28-Bsa4

Plasmid

#26103

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pAL149

Plasmid

#22275

Purpose

Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PhyB(1-908) (PHYB Mustard Weed)

Use

Synthetic Biology

Tags

mCherry-Kras4BCT (prenylation sequence)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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