161,601 results

1. pOTTC293 - pAAV EF1a V5-synuclein (WT)

   Plasmid

   #60057

   Purpose

   An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   alpha-synuclein (SNCA human, Human)
   

   Use

   AAV

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

   Plasmid

   #168101

   Purpose

   GFP and Flag tagged Nup62 with EPEA nanobody tag

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   GFP-Flag-Nup62-EPEA
   

   Use

   Tags

   EPEA, Flag, and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmEGFP-ADRA2A

   Plasmid

   #190753

   Purpose

   Expression of ADRA2A with mEGFP tag on N-terminus.

   Depositor

   Dave Piston

   Article

   Foust et al bioRxiv 2021.11.03.467169

   Insert

   ADRA2A (ADRA2A Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4 CoV-2 nsp1 3xFLAG

   Plasmid

   #176057

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CMV-PdCO-mScarlet

   Plasmid

   #198507

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HsGRK6-mVenus

   Plasmid

   #137780

   Purpose

   Visualization of GRK6

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Human GRK6 (GRK6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR U6-hTERTsh

   Plasmid

   #111385

   Purpose

   Knock down of hTERT transcript

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   shRNA C3 against hTERT
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyso-ExRai-AMPKAR

   Plasmid

   #192449

   Purpose

   ExRai-AMPKAR targeted to the lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   Lyso-ExRai-AMPKAR
   

   Use

   Tags

   Full-length LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220612

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-ymScarletI-SpHis5

    Plasmid

    #219844

    Purpose

    tagging vector to tag genes with ymScarletI and a HIS5 gene

    Depositor

    Bas Teusink

    Insert

    ymScarletI-SpHis5
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEGFP-C1-TULP3

    Plasmid

    #218741

    Purpose

    Expresses C-terminally EGFP-tagged TULP3 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    TULP3 (TULP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti6.3/V5-DEST-GFP

    Plasmid

    #40125

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EC67908

    Plasmid

    #211795

    Purpose

    Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    HptII, Cas12a, GR-GRF-GIF
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. VN-Sph1

    Plasmid

    #89755

    Purpose

    expresses human Synphilin fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

    Insert

    SNCAIP (SNCAIP Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIV270

    Plasmid

    #226181

    Purpose

    Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p (Komagataella phaffii)

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. UBQ:RUBY

    Plasmid

    #160909

    Purpose

    RUBY under the control of Ubiquitin promoter/marker for transformation

    Depositor

    Yunde Zhao

    Article

    He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

    Insert

    RUBY and UBIQUITIN promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC67844

    Plasmid

    #209514

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIB185

    Plasmid

    #90196

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-Luciferase-IRES-mCerulean

    Plasmid

    #75022

    Purpose

    Encodes the expression of Luciferase and mCerulean

    Depositor

    Saradhi Mallampati

    Insert

    Luciferase
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only