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pET30a-AlkB

Plasmid

#79050

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Tao Pan

Publication

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Publication

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pdCas9-DNMT3A-EGFP (ANV)

Plasmid

#71685

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFP-p150Glued

Plasmid

#36154

Depositor

David Stephens

Publication

Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

Insert

p150Glued (DCTN1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

see documentation

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLL7.0: Venus-iLID-Mito (From ActA)

Plasmid

#60413

Purpose

Mammalian Expression of Venus-iLID-Mito

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-Mito

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAV-EF1a-mCherry-IRES-Flpo

Plasmid

#55634

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has service

AAV Retrograde and AAV1

Inserts

mCherry
Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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mCardinal-C1

Plasmid

#54799

Purpose

Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Publication

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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YPet-N1

Plasmid

#54637

Purpose

Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Publication

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

YPet

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pCGN-HCF-1 fl

Plasmid

#53309

Purpose

plasmid contains human HCFC1 full length gene with HA and c-myc tags

Depositor

Winship Herr

Publication

Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Insert

HCFC1 (HCFC1 Human)

Tags

HA and c-myc

Expression

Mammalian

Promoter

CMV

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pFA6a-link-yomTagBFP2-SpHis5

Plasmid

#44839

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP2

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-28a-hPKM1

Plasmid

#44241

Depositor

Lewis Cantley, Matthew Vander Heiden

Publication

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M1 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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G6PD/pRK5

Plasmid

#41521

Depositor

Xiaolu Yang

Publication

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Insert

glucose-6-phosphate dehydrogenase (G6PD Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-SypHluorin 4x (S4x)

Plasmid

#37005

Depositor

Stephen Heinemann, Yongling Zhu

Publication

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin 4x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Four copies of pHluorin inserted at site 184-185

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HA-AMOT p130

Plasmid

#32821

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

E5D

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pDONR223-EGFR

Plasmid

#23935

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLM-vexGFP-Oct4

Plasmid

#22240

Purpose

2nd generation lentiviral vector

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

vexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FCK-Arch-GFP

Plasmid

#22217

Purpose

Lentiviral expression of Arch-GFP for optogenetic inhibition

Depositor

Edward Boyden

Publication

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Publication

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles