Addgene: A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag.

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A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag.
Yim WW, Yamamoto H, Mizushima N
Elife. 2022 Aug 8;11. pii: 78923. doi: 10.7554/eLife.78923. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
184899	pMRX-IP-HaloTag7-LC3	Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux
184900	pMRX-No-HaloTag7-LC3	Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux
184901	pMRX-No-HaloTag7-mGFP-LC3	Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux
184902	pMRX-No-HaloTag7-mGFP-LC3-mRFP	Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux
184903	pMRX-IB-HaloTag7-mGFP	Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux
184904	pMRX-IB-HaloTag7-mGFP-KDEL	Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity
184905	pMRX-IB-pSu9-HaloTag7-mGFP	Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

Antibodies from Article