RIP Cre-ER (DM#265)
(Plasmid #15029)

• Depositing Lab: Douglas Melton
• Publication: Dor et al Nature. 2004 May 6. 429(6987):41-6.

Backbone

• Vector backbone: modified pBluescript II KS
• Backbone size w/o insert (bp): 6700
• Vector type: Mammalian Expression, Cre/Lox ; for making transgenic constructs

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rat insulin promoter (RIP)
• Alt name: insulin promoter
• Alt name: ins2
• Species: R. norvegicus (rat)
• Insert Size (bp): 700
• Entrez Gene: Ins2 (a.k.a. CP-II)
• Tag / Fusion Protein:
  • Cre-ER (TM) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cre-ER(TM) was a gift from Andy McMahon.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.

The RIP–CreER construct was generated by fusing a 0.66-kilobase SmaI–HindIII fragment of the RIP2 promoter to a minimal hsp68 promoter, and placing the chimaeric promoter upstream of CreER(TM).

See Author's Map for more information.