Pdx1 Cre (DM#258)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15021||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression, Cre/Lox ; mouse transgenic
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePdx1 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)7000
Entrez GenePdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
/ Fusion Protein
- Cre (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion.
See Author's Map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Pdx1 Cre (DM#258) was a gift from Douglas Melton (Addgene plasmid # 15021 ; http://n2t.net/addgene:15021 ; RRID:Addgene_15021)
For your References section:Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Gu G, Dubauskaite J, Melton DA. Development. 2002 May . 129(10):2447-57. PubMed 11973276