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Pdx1 Cre (DM#258)
(Plasmid #15021)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15021 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, Cre/Lox ; mouse transgenic

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pdx1 promoter
  • Alt name
    pGD89
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7000
  • Entrez Gene
    Pdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
  • Tag / Fusion Protein
    • Cre (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Pdx1 promoter was a gift from Christopher V. E. Wright. pMC-Cre was a gift from Andrew McMahon.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion.

See Author's Map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pdx1 Cre (DM#258) was a gift from Douglas Melton (Addgene plasmid # 15021)
  • For your References section:

    Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Gu G, Dubauskaite J, Melton DA. Development. 2002 May . 129(10):2447-57. PubMed 11973276