Pdx1 Cre (DM#258)
(Plasmid #15021)

• Depositing Lab: Douglas Melton
• Publication: Gu et al Development. 2002 May . 129(10):2447-57.

Backbone

• Vector backbone: pBluescript II KS
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression, Cre/Lox ; mouse transgenic

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pdx1 promoter
• Alt name: pGD89
• Species: M. musculus (mouse)
• Insert Size (bp): 7000
• Entrez Gene: Pdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
• Tag / Fusion Protein:
  • Cre (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Pdx1 promoter was a gift from Christopher V. E. Wright. pMC-Cre was a gift from Andrew McMahon.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion.

See Author's Map for more information.