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Pdx1 Cre-ER (DM#255)
(Plasmid #15022)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 15022 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript II KS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, Cre/Lox ; mouse transgenic

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use Stbl3 to prevent recombination
  • Copy number
    High Copy


  • Gene/Insert name
    Pdx1 promoter
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Pdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)
  • Tag / Fusion Protein
    • Cre-ER(TM) (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cre-ER(TM) was a gift from Andy McMahon.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.

For Pdx-1-Cre-ER(TM), the coding region of the Cre-ER(TM) cDNA was directly fused to the starting ATG of the PDX1 protein by PCR. Three primers (p5, 5'-ttgaaacaagtgcaggtgttcg-3'; p75, 5'-gttgcatcgaccggtaatgcaggcaaattttggtgtacggtcagtaaattggacatggtggcagccggcact-3'; and p71, 5'-gttgcatcgaccggtaatgca-3') were used. First, p5 and p75 were used to amplify a 400 base pair fragment from the Pdx1 genomic DNA. Then this fragment was used together with p5 and p71 and the Cre-ER(TM) plasmid to obtain a fragment that has the 5' end coding region directly fused to the Pdx1 promoter. This fragment was digested with AgeI and ligated to the XhoI (blunt-ended)-AgeI double-digested pBSCre-ER(TM) that contains the full-length Cre-ER-coding region to give pGD19. Then a 2.2 kb insert was released from pGD19 by SmaI-SpeI digestion and ligated to SmaI-NotI digested pKSpdx-1SalI, and a SpeI-NotI digested PCR fragment that contains the SV40 polyadenylation signal to give pGD35. The 8 kb insert was released by SalI-NotI digestion and was used for pronucleus injection.

See Author's Map and article for more information.

SalI/SmaI digest should give 6.7, 1.5, 4 kb bands.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pdx1 Cre-ER (DM#255) was a gift from Douglas Melton (Addgene plasmid # 15022 ; ; RRID:Addgene_15022)
  • For your References section:

    Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Gu G, Dubauskaite J, Melton DA. Development. 2002 May . 129(10):2447-57. PubMed 11973276