165,888 results

1. pcDNA3-Drp1K38A

   Plasmid

   #45161

   Depositor

   Alexander van der Bliek, Richard Youle

   Article

   Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

   Insert

   Drp1 (DNM1L Human)
   

   Use

   Tags

   HA (AYPYDVPDY)

   Expression

   Mammalian

   Mutation

   K38A

   Promoter

   CMV

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT_TO_FlagHA_USP10

   Plasmid

   #127102

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   USP10 (USP10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-ASAP3-WPRE

   Plasmid

   #132331

   Purpose

   expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIX403-ccdB-Blast

   Plasmid

   #158560

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-eNme2-C-ABE8e

   Plasmid

   #185667

   Purpose

   Expresses eNme2-C-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV1)

   Viral Prep

   #26966-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

8. mEmerald-CD81-10

   Plasmid

   #54031

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   He et al PLoS Pathog. 2013;9(10):e1003701. doi: 1

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR223-ADRBK1

   Plasmid

   #23835

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ADRBK1 (GRK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PS6K-mcherry-iATPsnFR1.1

    Plasmid

    #227238

    Purpose

    Inducible ATP sensor for E. coli

    Depositor

    Fuzhong Zhang

    Article

    Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

    Insert

    iATPsnFR1.1
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAZ-SK

    Plasmid

    #73962

    Purpose

    self-killing gRNA plasmid pMAZ-SK

    Depositor

    Alex Nielsen

    Article

    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM496 (deletions) Zeo

    Plasmid

    #109301

    Purpose

    ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    Bxb1 attB site
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG1159 TOM20-ECFP-FIREmate

    Plasmid

    #196693

    Purpose

    Expresses TOM20-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    TOM20-ECFP-FIREmate
    

    Use

    Tags

    FIREmate

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-HA-eLACCO2.1-NGR

    Plasmid

    #208023

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. p177bp-LacOx50-Hyg

    Plasmid

    #210213

    Purpose

    Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    50xLacO repeats derived from: PMID: 12864855.
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLIB

    Plasmid

    #80610

    Purpose

    pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5F-TACE

    Plasmid

    #31713

    Purpose

    Mammalian expression of human TACE (ADAM17)

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TACE (ADAM17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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