A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences.
Gleichman AJ, Kawaguchi R, Sofroniew MV, Carmichael ST
Nat Commun. 2023 Nov 16;14(1):7426. doi: 10.1038/s41467-023-42746-w. PubMed Article
Gleichman AJ, Kawaguchi R, Sofroniew MV, Carmichael ST
Nat Commun. 2023 Nov 16;14(1):7426. doi: 10.1038/s41467-023-42746-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196410 | AAV-GfaABC1D-Cre-4x6T | Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196411 | AAV-GfaABC1D-ERCreER-4x6T | Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196412 | AAV-GfaABC1D-iCreV-4x6T | Astrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196413 | AAV-GfaABC1D-DreO-4x6T | Astrocytic expression of Dre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196414 | AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE | Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196415 | AAV-GfaABC1D-lck-smMyc-4x6T-WPRE | Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196416 | AAV-GfaABC1D-lck-smV5-4x6T-WPRE | Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196417 | AAV-GfaABC1D-MCS--4x6T-WPRE | Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette |
| 196418 | AAV-CAG-flex-GFP-4x6T | Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette |
| 196419 | AAV-CAG-flex-lck-smV5-4x6T | Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette |
| 196420 | AAV-CAG-flex-MCS--4x6T | Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette |
| 196421 | AAV-CAG-dDIO-lck-smMyc-4x6T | Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette |
| 196422 | AAV-CAG-dDIO-MCS--4x6T | Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette |
| 196423 | AAV-CAG-flex-lck-smV5 | Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity |