Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #196420)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196420 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    AAV-FLEX (Addgene 28304)
  • Backbone manufacturer
    Scott Sternson
  • Backbone size (bp) 5077
  • Modifications to backbone
    Added 4x6T miRNA targeting cassette for astrocyte selectivity
  • Vector type
    Mammalian Expression, AAV, Cre/Lox ; Astrocyte-selective
  • Promoter CAG

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTTCGGCTTCTGGCGTGTGACC
  • 3′ sequencing primer ttaaagcagcgtatccacat
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    AAV-CAG-flex-MCS--4x6T was created using pAAV-FLEX-GFP (Addgene 28304) provided by Ed Boyden.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CAG-flex-MCS--4x6T was a gift from Stanley Thomas Carmichael (Addgene plasmid # 196420 ; ; RRID:Addgene_196420)
  • For your References section:

    A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences. Gleichman AJ, Kawaguchi R, Sofroniew MV, Carmichael ST. Nat Commun. 2023 Nov 16;14(1):7426. doi: 10.1038/s41467-023-42746-w. 10.1038/s41467-023-42746-w PubMed 37973910