PurposeFluorescent reporter for calcium signaling
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52925||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
|AAV5||52925-AAV5||Virus (100 µL at titer ≥ 7×10¹² vg/mL)|
This material is available to academics and nonprofits only.
Vector backbonepZac 2.1
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Information for AAV5 (Catalog # 52925-AAV5) ( Back to top )
Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA.gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.
- Volume 100 µL
- Titer ≥ 7×10¹² vg/mL
- Pricing $350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- Storage Store at -80℃. Thaw just before use and keep on ice.
- Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- Buffer PBS + 0.001% Pluronic F-68 + 200 mM NaCl
- Serotype AAV5
- Purification Iodixanol gradient ultracentrifugation
Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide
Terms and Licenses
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZac2.1 gfaABC1D-cyto-GCaMP6f was a gift from Baljit Khakh (Addgene plasmid # 52925 ; http://n2t.net/addgene:52925 ; RRID:Addgene_52925)
For viral preps, please replace (Addgene plasmid # 52925) in the above sentence with: (Addgene viral prep # 52925-AAV5)
For your References section:Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway. Haustein MD, Kracun S, Lu XH, Shih T, Jackson-Weaver O, Tong X, Xu J, Yang XW, O'Dell TJ, Marvin JS, Ellisman MH, Bushong EA, Looger LL, Khakh BS. Neuron. 2014 Apr 16;82(2):413-29. doi: 10.1016/j.neuron.2014.02.041. 10.1016/j.neuron.2014.02.041 PubMed 24742463