167,924 results

1. PAC_E83_LOV_eGFP

   Plasmid

   #231574

   Purpose

   This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYFJ84

   Plasmid

   #227644

   Purpose

   Vibrio harveyi acyl-ACP synthetase

   Depositor

   John Cronan

   Article

   Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

   Insert

   Acyl-Acyl carrier protein synthetase
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmEGFP-N1

   Plasmid

   #217486

   Purpose

   Expression of monomeric Enhanced Green Fluorescent Protein

   Depositor

   Bertrand Collet

   Article

   Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

   Insert

   mEGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-Leup

   Plasmid

   #213154

   Purpose

   Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

   Depositor

   Jason Crawford

   Article

   Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

   Insert

   Leupeptin Protease Inhibitor Pathway
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

   Plasmid

   #210767

   Purpose

   Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   DRSR 514-517 to AS; 557 R to A; multiple silent m…

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiT3.1-N_RBBP4:1-425

   Plasmid

   #211037

   Purpose

   Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RBBP4:M1-S425 (RBBP4 Human)
   

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE80l BCB

   Plasmid

   #196472

   Purpose

   Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   BCB
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

   Plasmid

   #175256

   Purpose

   SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

   Depositor

   Johanna Ivaska

   Article

   Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

   Insert

   SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-kanMX6-P3nmt1-mECitrine

   Plasmid

   #105151

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

   Insert

   kanMX6-P3nmt1-mECitrine
   

   Tags

   3nmt1-mECitrine

   Expression

   Bacterial and Yeast

   Mutation

   F64L, Q69M, A206K

   Promoter

   3nmt1

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-kanMX6-P81nmt1-mECitrine

    Plasmid

    #105153

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

    Insert

    kanMX6-P81nmt1-mECitrine
    

    Tags

    81nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    81nmt1

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-kanMX6-P41nmt1-mECitrine

    Plasmid

    #105152

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    kanMX6-P41nmt1-mECitrine
    

    Tags

    41nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    41nmt1

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. jk68

    Plasmid

    #87020

    Purpose

    genomic Sindbis virus plasmid for use in SYNseq PLA screen

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    SNAP-Nlg1AB-4xλN(i)
    mCherry
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Expression

    Mammalian

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBP-lacZ

    Plasmid

    #72948

    Purpose

    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - Bioparts
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pADH99

    Plasmid

    #90979

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pADH110

    Plasmid

    #90982

    Purpose

    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    NAT 2 of 2
    pSNR52
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. eGFP-ova-C1

    Plasmid

    #163524

    Purpose

    Expresses both eGFP and chicken ovalbumin in mammalian cells

    Depositor

    Thomas Weber

    Insert

    enhanced-GFP-chicken ovalbumin tandem
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

    Plasmid

    #233628

    Purpose

    Expression of bicistronic reporter with TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5_mEGFP-NPM1

    Plasmid

    #237667

    Purpose

    For overexpression of mEGFP-NPM1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. PEmax-mRNA

    Plasmid

    #204472

    Purpose

    plasmid for PEmax mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

    Insert

    Prime editor
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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