316,190 results

1. PX393 Gluconacetobacter diazotrophicus Cas9

   Plasmid

   #68330

   Purpose

   Gluconacetobacter diazotrophicus Cas9

   Depositor

   Feng Zhang

   Insert

   GdCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJExpress414_GmPOPB-D661A

   Plasmid

   #92236

   Purpose

   Express D661A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-D661A mutant
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Aspartate 661 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJExpress414_GmPOPB-D661A

   Plasmid

   #92236

   Purpose

   Express D661A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-D661A mutant
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Aspartate 661 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNUTN6His Y426F/Y517F hTFNG

   Plasmid

   #70084

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNUTN6His Y426F/Y517F hTFNG

   Plasmid

   #70084

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNUT N6His Y95F/Y188F hTFNG

   Plasmid

   #69601

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNUT N6His Y95F/Y188F hTFNG

   Plasmid

   #69601

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pH-PCE

   Plasmid

   #242427

   Purpose

   for chromosome editing

   Depositor

   Caixia Gao

   Article

   Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

   Insert

   pGSPE-epeg, PE, cm24Cre
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH-SpRY-PCE

   Plasmid

   #242428

   Purpose

   for chromosome editing

   Depositor

   Caixia Gao

   Article

   Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

   Insert

   pGSPE-epeg, SpRY-PE, cm24Cre
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid

    #226429

    Purpose

    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

    Depositor

    Alon Goren

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-Hia5-OPT1

    Plasmid

    #231968

    Purpose

    Expresses DNA adenine N6-methyltransferase Hia5 in Escherichia coli (E.coli) T7 Express lysY/Iq

    Depositor

    Schahram Akbarian

    Article

    Peter et al Cell Rep Methods. 2024 Nov 25:100911. do

    Insert

    DNA adenine N6-methyltransferase Hia5
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SpRY_ATH51-IP

    Plasmid

    #235991

    Purpose

    Expresses SpRY_ATH51 in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    SpRY_ATH51
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    ectopic insertion of 7 amino acids of PID turn-he…

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pR-miRFP670

    Plasmid

    #228612

    Purpose

    Mammalian expression of miRFP670, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Anti-6xHis [N144/14-rat IgG2a] - Chimeric

    Plasmid

    #227037

    Purpose

    Mammalian expression plasmid of anti-6xHis rat IgG2a R-mAb. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-6xHis recombinant mouse monoclonal antibody.
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pR-mTagBFP2

    Plasmid

    #228596

    Purpose

    Mammalian expression of mTagBFP2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pR-mRuby3

    Plasmid

    #228609

    Purpose

    Mammalian expression of mRuby3, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SL2 GFP H2B

    Plasmid

    #227717

    Purpose

    Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Haosheng Sun, Oliver Hobert

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    SL2 GFP H2B (nlp-51 Nematode)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME-Luciferase-P2A-H2A-mCherry (JDW 926)

    Plasmid

    #224488

    Purpose

    Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LT3GEPIR-CDK1shRNA1

    Plasmid

    #220569

    Purpose

    To inducibly knockdown CDK1 expression

    Depositor

    Sean Lee

    Insert

    CDK1 shRNA1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LT3GEPIR-CDK1shRNA1

    Plasmid

    #220569

    Purpose

    To inducibly knockdown CDK1 expression

    Depositor

    Sean Lee

    Insert

    CDK1 shRNA1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge