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Differential activation of CREB by Ca2+/calmodulin-dependent protein kinases type II and type IV involves phosphorylation of a site that negatively regulates activity.

Sun P, Enslen H, Myung PS, Maurer RA
Genes Dev. 1994 Nov 1;8(21):2527-39. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
45062pRSV-CaMKIVExpression of CaMKIV
45063pRSV-CaMKIV(1-313)Expression of constit active CaMKIV
45064pRSV-CaMKIIExpression of CaMKII
45065pRSV-CaMKII(1-290)Expression of constit active CaMKII
467565xGAL4-TATA-luciferaseLuciferase reporter gene for factors with GAL4 DNA binding domains
46769pcDNAI-GAL4-CREBExpression of GAL4 fusion to wild type CREB
46770pcDNAI-GAL4-CREB-S133AExpression of GAL4 fusion to CREB-S133A
46771pcDNAI-GAL4-CREB-S142AExpression of GAL4 fusion to CREB-S142A

Antibodies from Article