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Addgene

pcDNAI-GAL4-CREB-S133A
(Plasmid #46770)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46770 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNAI-AMP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4767
  • Total vector size (bp) 6206
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GAL4(1-147)-CREB-S133A
  • Species
    R. norvegicus (rat), S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1500
  • Mutation
    Changed CREB serine 133 to Alanine
  • GenBank ID
    NM_001184062.1 NM_134443.1
  • Entrez Gene
    Creb1 (a.k.a. Creb)
  • Entrez Gene
    GAL4 (a.k.a. YPL248C, GAL81)
  • Promoter CMV
  • Tag / Fusion Protein
    • GAL4 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer t7
  • 3′ sequencing primer sp6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The rat CREB coding sequence was obtained by PCR from mRNA. The GAL4(1-147) coding sequence was obtained from PG424 (Sadowski & Ptashne, Nucleic Acids Res. 17:7539, 1989) which was provided by Dr. Ptashne.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAI-GAL4-CREB-S133A was a gift from Richard Maurer (Addgene plasmid # 46770 ; http://n2t.net/addgene:46770 ; RRID:Addgene_46770)
  • For your References section:

    Differential activation of CREB by Ca2+/calmodulin-dependent protein kinases type II and type IV involves phosphorylation of a site that negatively regulates activity. Sun P, Enslen H, Myung PS, Maurer RA. Genes Dev. 1994 Nov 1;8(21):2527-39. 10.1101/gad.8.21.2527 PubMed 7958915