Improving the photostability of bright monomeric orange and red fluorescent proteins.
Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY
Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4. PubMed Article
Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY
Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 54531 | mOrange2-pBAD | Localization: Protein Expression Vector , Excitation: 549, Emission: 565 |
| 54536 | mApple-pBAD | Localization: Protein Expression Vector , Excitation: 568, Emission: 592 |
| 54567 | mApple-N1 | Localization: N1 Cloning Vector, Excitation: 568, Emission: 592 |
| 54568 | mOrange2-N1 | Localization: N1 Cloning Vector, Excitation: 549, Emission: 565 |
| 54650 | mOrange2-C1 | Localization: C1 Cloning Vector, Excitation: 549, Emission: 565 |
| 57867 | mKO2-Cx43-7 | Localization: Gap Junctions, Excitation: 551, Emission: 565 |
| 57944 | mOrange2-Alpha-Actinin-19 | Localization: Alpha Actinin, Excitation: 549, Emission: 565 |
| 57947 | mOrange2-Clathrin-15 | Localization: Clathrin Vesicles, Excitation: 549, Emission: 565 |
| 57949 | mOrange2-Cx26-7 | Localization: Gap Junctions, Excitation: 549, Emission: 565 |
| 57951 | mOrange2-Cx43-7 | Localization: Gap Junctions, Excitation: 549, Emission: 565 |
| 57953 | mOrange2-EB3-7 | Localization: MT End Binding Protein, Excitation: 549, Emission: 565 |
| 57955 | mOrange2-Endo-14 | Localization: Endosomes, Excitation: 549, Emission: 565 |
| 57957 | mOrange2-Fibrillarin-7 | Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565 |
| 57959 | mOrange2-Golgi-7 | Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565 |
| 57965 | mOrange2-Keratin-17 | Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565 |
| 57966 | mOrange2-LaminB1-10 | Localization: Nuclear Envelope, Excitation: 549, Emission: 565 |
| 57968 | mOrange2-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 549, Emission: 565 |
| 57969 | mOrange2-Mito-7 | Localization: Mitochondria, Excitation: 549, Emission: 565 |
| 57970 | mOrange2-Paxillin-22 | Localization: Focal Adhesions, Excitation: 549, Emission: 565 |
| 57974 | mOrange2-Tubulin-6 | Localization: Microtubules, Excitation: 549, Emission: 565 |
| 57977 | mOrange2-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 549, Emission: 565 |
| 57978 | mOrange2-Vinculin-23 | Localization: Focal Adhesions, Excitation: 549, Emission: 565 |
| 58001 | mTagRFP-T-Actin-7 | Localization: Actin, Excitation: 555, Emission: 584 |
| 58005 | mTagRFP-T-Clathrin-15 | Localization: Clathrin Vesicles, Excitation: 555, Emission: 584 |
| 58007 | mTagRFP-T-Cx26-7 | Localization: Gap Junctions, Excitation: 555, Emission: 584 |
| 58009 | mTagRFP-T-Cx43-7 | Localization: Gap Junctions, Excitation: 555, Emission: 584 |
| 58016 | mTagRFP-T-Fibrillarin-7 | Localization: Nucleoli, Excitation: 555, Emission: 584 |
| 58017 | mTagRFP-T-Gamma-Tubulin-17 | Localization: Centrosomes, Excitation: 555, Emission: 584 |
| 58018 | mTagRFP-T-Golgi-7 | Localization: Golgi Complex, Excitation: 555, Emission: 584 |
| 58019 | mTagRFP-T-H2B-6 | Localization: Nucleus/Histones, Excitation: 555, Emission: 584 |
| 58020 | mTagRFP-T-LaminB1-10 | Localization: Nuclear Envelope, Excitation: 555, Emission: 584 |
| 58022 | mTagRFP-T-Lysosomes-20 | Localization: Lysosome Membrane, Excitation: 555, Emission: 584 |
| 58024 | mTagRFP-T-Paxillin-22 | Localization: Focal Adhesions, Excitation: 555, Emission: 584 |
| 58029 | mTagRFP-T-Vimentin-7 | Localization: Intermediate Filaments, Excitation: 555, Emission: 584 |
| 58030 | mTagRFP-T-Vinculin-23 | Localization: Focal Adhesions, Excitation: 555, Emission: 584 |
| 58086 | tdTomato-Cx43-7 | Localization: Gap Junctions, Excitation: 554, Emission: 581 |
| 58099 | tdTomato-Gamma-Tubulin-17 | Localization: Centrosomes, Excitation: 554, Emission: 581 |
| 91772 | pBAD mApple | Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence. |