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Improving the photostability of bright monomeric orange and red fluorescent proteins.
Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY
Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
54531mOrange2-pBADLocalization: Protein Expression Vector , Excitation: 549, Emission: 565
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54536mApple-pBADLocalization: Protein Expression Vector , Excitation: 568, Emission: 592
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54567mApple-N1Localization: N1 Cloning Vector, Excitation: 568, Emission: 592
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54568mOrange2-N1Localization: N1 Cloning Vector, Excitation: 549, Emission: 565
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54650mOrange2-C1Localization: C1 Cloning Vector, Excitation: 549, Emission: 565
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57867mKO2-Cx43-7Localization: Gap Junctions, Excitation: 551, Emission: 565
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57944mOrange2-Alpha-Actinin-19Localization: Alpha Actinin, Excitation: 549, Emission: 565
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57947mOrange2-Clathrin-15Localization: Clathrin Vesicles, Excitation: 549, Emission: 565
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57949mOrange2-Cx26-7Localization: Gap Junctions, Excitation: 549, Emission: 565
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57951mOrange2-Cx43-7Localization: Gap Junctions, Excitation: 549, Emission: 565
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57953mOrange2-EB3-7Localization: MT End Binding Protein, Excitation: 549, Emission: 565
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57955mOrange2-Endo-14Localization: Endosomes, Excitation: 549, Emission: 565
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57957mOrange2-Fibrillarin-7Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565
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57959mOrange2-Golgi-7Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565
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57965mOrange2-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565
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57966mOrange2-LaminB1-10Localization: Nuclear Envelope, Excitation: 549, Emission: 565
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57968mOrange2-Lysosomes-20Localization: Lysosome Membrane, Excitation: 549, Emission: 565
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57969mOrange2-Mito-7Localization: Mitochondria, Excitation: 549, Emission: 565
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57970mOrange2-Paxillin-22Localization: Focal Adhesions, Excitation: 549, Emission: 565
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57974mOrange2-Tubulin-6Localization: Microtubules, Excitation: 549, Emission: 565
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57977mOrange2-Vimentin-7Localization: Intermediate Filaments, Excitation: 549, Emission: 565
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57978mOrange2-Vinculin-23Localization: Focal Adhesions, Excitation: 549, Emission: 565
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58001mTagRFP-T-Actin-7Localization: Actin, Excitation: 555, Emission: 584
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58005mTagRFP-T-Clathrin-15Localization: Clathrin Vesicles, Excitation: 555, Emission: 584
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58007mTagRFP-T-Cx26-7Localization: Gap Junctions, Excitation: 555, Emission: 584
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58009mTagRFP-T-Cx43-7Localization: Gap Junctions, Excitation: 555, Emission: 584
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58016mTagRFP-T-Fibrillarin-7Localization: Nucleoli, Excitation: 555, Emission: 584
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58017mTagRFP-T-Gamma-Tubulin-17Localization: Centrosomes, Excitation: 555, Emission: 584
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58018mTagRFP-T-Golgi-7Localization: Golgi Complex, Excitation: 555, Emission: 584
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58019mTagRFP-T-H2B-6Localization: Nucleus/Histones, Excitation: 555, Emission: 584
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58020mTagRFP-T-LaminB1-10Localization: Nuclear Envelope, Excitation: 555, Emission: 584
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58022mTagRFP-T-Lysosomes-20Localization: Lysosome Membrane, Excitation: 555, Emission: 584
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58024mTagRFP-T-Paxillin-22Localization: Focal Adhesions, Excitation: 555, Emission: 584
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58029mTagRFP-T-Vimentin-7Localization: Intermediate Filaments, Excitation: 555, Emission: 584
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58030mTagRFP-T-Vinculin-23Localization: Focal Adhesions, Excitation: 555, Emission: 584
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58086tdTomato-Cx43-7Localization: Gap Junctions, Excitation: 554, Emission: 581
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58099tdTomato-Gamma-Tubulin-17Localization: Centrosomes, Excitation: 554, Emission: 581
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91772pBAD mAppleBacterial expression of a fluorescent protein, monomeric Apple (mApple)
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