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Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.
Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B
Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51023pSLQ1658-dCas9-EGFPTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)
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51024pSLQ1651-sgTelomere(F+E)Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres
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51025pSLQ1661-sgMUC4-E3(F+E)Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3
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