N-cadherin in pCCL-c-MNDU3c-PGK-EGFP
(Plasmid #38153)

• Depositing Lab: Nora Heisterkamp
• Publication: Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2007 Mar 29.

Backbone

• Vector backbone: pCCL-c-MNDU3c-(X2)-Pgk-EGFP
• Backbone manufacturer: Don Kohn
• Backbone size w/o insert (bp): 7900
• Total vector size (bp): 12000
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: N-cadherin
• Species: H. sapiens (human)
• Insert Size (bp): 4100
• GenBank ID: NM_001792
• Entrez Gene: CDH2 (a.k.a. ACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD)
• Promoter: MNDU3c = LTR promoter for U3 region of myeloproliferative sarcoma retrovirus MND

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: MSCV_rev
• 3′ sequencing primer: EGFP-N

Gene/Insert 2

• Gene/Insert name: EGFP
• Promoter: PGK

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: mPGK-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: purchased clone from Origene human N-cadherin in pCMV6-XL6
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Bin Zhang. Vector was digested with EcoRI and the site blunted by filling in with T4 DNA polymerase. N-cadherin was isolated as a blunt-end fragment by digesting N-cadherin in pCMV6-XL with BsrBI (in front of the ATG) and with SmaI (in the vector). Has good N-cadherin expression. This vector expresses EGFP from the PGK promoter and non-tagged human N-cadherin.