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A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.
Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
BMC Biotechnol. 2014 Sep 25;14(1):84.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
59382pT2SCTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.
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59383pT2SKTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.
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59384pT2STTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.
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59385pT2SCbTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.
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59386pSLTSExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.
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