233,568 results

1. pBJ5 mCnA alpha

   Plasmid

   #17871

   Depositor

   Jerry Crabtree

   Article

   Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

   Insert

   CnA alpha (Ppp3ca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRepair-SGFP2-CTNNB1

   Plasmid

   #153430

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   SGFP2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. R777-E233 Hs.RASAL1

   Plasmid

   #70517

   Purpose

   Gateway ORF clone of human RASAL1 [NM_001193521.1] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RASAL1 (RASAL1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   R331H

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGHE-PdNIP2-1

   Plasmid

   #108447

   Purpose

   PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

   Depositor

   Richard Bélanger

   Insert

   PdNIP2-1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIRT4.23

   Plasmid

   #13737

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   SIRT4 (SIRT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pHCMV_SARS-CoV_S

   Plasmid

   #207306

   Purpose

   Mammalian expression of Human SARS coronavirus S protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SARS-CoV_S (S Human SARS coronavirus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EGFP-Cre

   Plasmid

   #86805

   Purpose

   Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter

   Depositor

   Amy Lasek

   Article

   Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02

   Insert

   Cre recombinase
   

   Use

   Cre/Lox and Lentiviral

   Tags

   EGFP and nuclear localization signal: PKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX459-CTNNB1-ATG

   Plasmid

   #153429

   Purpose

   Expresses a gRNA that overlaps the startcodon of human CTNNB1

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 gRNA (CTNNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid

    #219564

    Purpose

    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    3` CC: Hygro – GFP-N – loxP – mScarlet
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5` CC: Puro – loxP – GFP-C

    Plasmid

    #219563

    Purpose

    5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    hPGK-promoter_PuroR_2A_loxP_GFP-C
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-IRES-Blast

    Plasmid

    #85133

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMU-MITr

    Plasmid

    #61198

    Purpose

    Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    ScCOX4sp-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-1b-S2

    Plasmid

    #128590

    Purpose

    Expresses tagless S2 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S2 (rpsB E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. BL21ΔABC

    Bacterial Strain

    #102266

    Purpose

    ompA, lamB, ompC triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

16. 3349 pSFFV-neo Bcl-2

    Plasmid

    #8769

    Depositor

    Stanley Korsmeyer

    Article

    Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-myc-Miro1

    Plasmid

    #47888

    Purpose

    Expresses myc tagged Miro1

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEasy Flox

    Plasmid

    #11725

    Depositor

    Klaus Rajewsky

    Article

    Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

    Insert

    pEasyFlox
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExp-Skp

    Plasmid

    #129241

    Purpose

    Protein expression in E.coli

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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