175,393 results
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Plasmid#216814PurposeAspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmNeonGreen
TagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)
Plasmid#221552PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR5-DuET
Plasmid#213380PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_WDR54:1-334
Plasmid#210931PurposeInsect expression of full-length human WDR54. N-terminal 6X His tag with TEV cleavage.DepositorInsertWDR54:M1-V334 (WDR54 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEntry_HCoV-229E-nsp9
Plasmid#168958PurposeGateway-compatible Entry vectorDepositorInsertHCoV-229E-nsp9 (HCoV229Egp1 )
UseGateway CloningAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I32 pLBD16 GG0-GG1
Plasmid#195903PurposeLevel 0 pLBD16 promoter with GG0 and GG1 golden gate linkersDepositorInsertpLBD16
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD226-Fc(DAPA)-AviTag-6xHis
Plasmid#156688PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD226 (CD226 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2357_Tier3(PB)-TagBFP_BlastR
Plasmid#169656PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and BlastR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-CPL2/pET28a
Plasmid#172095PurposecDNA of CER11/CPL2 cloned into pET28a for protein expressionDepositorAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPaint-mNeon-F-gB-PuroR [M1G]
Plasmid#171803PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-gB-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-mouse_min2
Plasmid#173965PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and mouse minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseMutationNonePromoterSV40 promoterAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUL33
Plasmid#135103PurposeCodon-optimized HSV-1 UL33 with N-terminal HAStrep tagDepositorInsertHSV-1 UL33 codon optimized
TagsHA-Strep-StrepExpressionMammalianAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sph1-VC
Plasmid#89756Purposeexpresses human Synphilin fused to the C-terminal part of VenusDepositorAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-tagged NRP1 (human)
Plasmid#213068PurposeExpress myc-NRP1 in mammalian cellsDepositorInsertneuropilin 1 (NRP1 Human)
TagsMycExpressionMammalianMutationN-terminal myc tag was introduced by a three-step…PromoterT7Available SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-MVP
Plasmid#204543PurposeExpresses EGFP-tagged MVP in mammalian cellsDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIgG1-2.1
Plasmid#225831PurposeMouse IgG1 chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG- and Strep2-tagged monoclonal antibodies for use as tag-specific ELISA standards.DepositorInsertMouse anti-CGG, IgG1 heavy chain
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-CAG-hM4Di-P2A-dTomato
Plasmid#202037PurposeAAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomatoDepositorInserthM4Di
UseAAVTagsdTomatoPromoterCAGAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-eGFP-Flag-SETX
Plasmid#218838PurposeMammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.DepositorAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only