Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET-28a-hPKM1
(Plasmid #44241)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44241 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pyruvate Kinase M1
  • Alt name
    PKM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Entrez Gene
    PKM (a.k.a. CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Promoter Primer
  • 3′ sequencing primer T7 Terminator Primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a-hPKM1 was a gift from Lewis Cantley & Matthew Vander Heiden (Addgene plasmid # 44241 ; http://n2t.net/addgene:44241 ; RRID:Addgene_44241)
  • For your References section:

    Pyruvate kinase M2 is a phosphotyrosine-binding protein. Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC. Nature. 2008 Mar 13;452(7184):181-6. doi: 10.1038/nature06667. 10.1038/nature06667 PubMed 18337815