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pEGFP-C1-PKM2
(Plasmid #64698)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64698 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6310
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PKM2
  • Alt name
    PKM transcript variant 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1610
  • GenBank ID
    NM_002654
  • Entrez Gene
    PKM (a.k.a. CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GATCACATGGTCCTGCTGGAG
  • 3′ sequencing primer TGCATTCATTTTATGTTTCAGGTTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-PKM2 was a gift from Axel Ullrich (Addgene plasmid # 64698 ; http://n2t.net/addgene:64698 ; RRID:Addgene_64698)
  • For your References section:

    Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death. Stetak A, Veress R, Ovadi J, Csermely P, Keri G, Ullrich A. Cancer Res. 2007 Feb 15;67(4):1602-8. 10.1158/0008-5472.CAN-06-2870 PubMed 17308100